BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4775

Title: Solution structure of the spindle assembly checkpoint protein human MAD2   PubMed: 10700282

Authors: Luo, X.; Fang, G.; Coldiron, M.; Lin, Y.; Yu, H.

Citation: Luo, X.; Fang, G.; Coldiron, M.; Lin, Y.; Yu, H.; Kirschner, M.; Wagner, G.. "Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20"  Nat. Struct. Biol. 7, 224-229 (2000).

Assembly members:
SPINDLE ASSEMBLY CHECKPOINT PROTEIN, polymer, 187 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SPINDLE ASSEMBLY CHECKPOINT PROTEIN: GSITLRGSAEIVAEFFSFGI NSILYQRGIYPSETFTRVQK YGLTLLVTTDLELIKYLNNV VEQLKDWLYKCSVQKLVVVI SNIESGEVLERWQFDIECDK TAKDDSAPREKSQKAIQDEI RSVIRQITATVTFLPLLEVS CSFDLLIYTDKDLVVPEKWE ESGPQFITNSEEVRLRSFTT TIHKVNS

Data sets:
Data typeCount
1H chemical shifts1220
13C chemical shifts601
15N chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MAD21

Entities:

Entity 1, MAD2 187 residues - Formula weight is not available

1   GLYSERILETHRLEUARGGLYSERALAGLU
2   ILEVALALAGLUPHEPHESERPHEGLYILE
3   ASNSERILELEUTYRGLNARGGLYILETYR
4   PROSERGLUTHRPHETHRARGVALGLNLYS
5   TYRGLYLEUTHRLEULEUVALTHRTHRASP
6   LEUGLULEUILELYSTYRLEUASNASNVAL
7   VALGLUGLNLEULYSASPTRPLEUTYRLYS
8   CYSSERVALGLNLYSLEUVALVALVALILE
9   SERASNILEGLUSERGLYGLUVALLEUGLU
10   ARGTRPGLNPHEASPILEGLUCYSASPLYS
11   THRALALYSASPASPSERALAPROARGGLU
12   LYSSERGLNLYSALAILEGLNASPGLUILE
13   ARGSERVALILEARGGLNILETHRALATHR
14   VALTHRPHELEUPROLEULEUGLUVALSER
15   CYSSERPHEASPLEULEUILETYRTHRASP
16   LYSASPLEUVALVALPROGLULYSTRPGLU
17   GLUSERGLYPROGLNPHEILETHRASNSER
18   GLUGLUVALARGLEUARGSERPHETHRTHR
19   THRILEHISLYSVALASNSER

Samples:

sample_1: SPINDLE ASSEMBLY CHECKPOINT PROTEIN, [U-15N; U-13C; U-2H], 1.2 mM; sodium phosphate 50 mM; KCl 300 mM; DTT 5 mM; NaN3 0.04%; H2O 90%; D2O 10%

sample_2: SPINDLE ASSEMBLY CHECKPOINT PROTEIN, [U-15N; U-13C; U-60% 2H], 1.4 mM; sodium phosphate 50 mM; KCl 300 mM; DTT 5 mM; NaN3 0.04%; H2O 90%; D2O 10%

sample_3: SPINDLE ASSEMBLY CHECKPOINT PROTEIN, [U-15N], 1.5 mM; sodium phosphate 50 mM; KCl 300 mM; DTT 5 mM; NaN3 0.04%; H2O 90%; D2O 10%

sample_4: SPINDLE ASSEMBLY CHECKPOINT PROTEIN, [U-10% 13C], 1.6 mM; sodium phosphate 50 mM; KCl 300 mM; DTT 5 mM; NaN3 0.04%; H2O 90%; D2O 10%

sample_5: SPINDLE ASSEMBLY CHECKPOINT PROTEIN 1.2 mM; sodium phosphate 50 mM; KCl 300 mM; DTT 5 mM; NaN3 0.04%; D2O 100%

sample_cond_1: pH: 6.8; temperature: 303 K; ionic strength: 300 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-NOESYnot availablenot availablenot available
3D 13C-NOESYnot availablenot availablenot available
3D HCCH TOCSYnot availablenot availablenot available
3D 15N-TOCSYnot availablenot availablenot available
3D H(CC)(CO)NHnot availablenot availablenot available
3D (H)C(C)(CO)NHnot availablenot availablenot available

Software:

X-PLOR v3.1 - structure calculation

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB63410 BAD97153 BAE01009 BAG36562 BAI46856
EMBL CAA03943
GB AAC50781 AAC52060 AAH00356 AAH05945 AAH70283
REF NP_001178442 NP_001182742 NP_001270194 NP_002349 XP_001084989
SP Q13257
TPG DAA28937