BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4778

Title: Backbone 1HN, 15N and 13C shifts for GMPPNP-loaded Cdc42 from Candida albicans   PubMed: 12815258

Authors: Osborne, Michael; Stevens, Willem; Ping, Xu; Ni, Feng

Citation: Osborne, Michael; Su, Z.; Sridaran, V.; Ni, Feng. "Efficient expression of isotopically labeled peptides for high resolution NMR studies: application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans "  J. Biomol. NMR 26, 317-326 (2003).

Assembly members:
Cell division control protein 42, polymer, 181 residues, Formula weight is not available
5p-guanosyl-imido-triphosphate, non-polymer, 522.196 Da.

Natural source:   Common Name: Yeast   Taxonomy ID: 5476   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Candida albicans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cell division control protein 42: GSHMQTIKCVVVGDGAVGKT CLLISYTTSKFPADYVPTVF DNYAVTVMIGDEPFTLGLFD TAGQEDYDRLRPLSYPSTDV FLVCFSVISPASFENVKEKW FPEVHHHCPGVPIIIVGTQT DLRNDDVILQRLHRQKLSPI TQEQGEKLAKELRAVKYVEC SALTQRGLKTVFDEAIVAAL E

Data sets:
Data typeCount
1H chemical shifts140
13C chemical shifts253
15N chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc421
2GNP2

Entities:

Entity 1, Cdc42 181 residues - Formula weight is not available

1   GLYSERHISMETGLNTHRILELYSCYSVAL
2   VALVALGLYASPGLYALAVALGLYLYSTHR
3   CYSLEULEUILESERTYRTHRTHRSERLYS
4   PHEPROALAASPTYRVALPROTHRVALPHE
5   ASPASNTYRALAVALTHRVALMETILEGLY
6   ASPGLUPROPHETHRLEUGLYLEUPHEASP
7   THRALAGLYGLNGLUASPTYRASPARGLEU
8   ARGPROLEUSERTYRPROSERTHRASPVAL
9   PHELEUVALCYSPHESERVALILESERPRO
10   ALASERPHEGLUASNVALLYSGLULYSTRP
11   PHEPROGLUVALHISHISHISCYSPROGLY
12   VALPROILEILEILEVALGLYTHRGLNTHR
13   ASPLEUARGASNASPASPVALILELEUGLN
14   ARGLEUHISARGGLNLYSLEUSERPROILE
15   THRGLNGLUGLNGLYGLULYSLEUALALYS
16   GLULEUARGALAVALLYSTYRVALGLUCYS
17   SERALALEUTHRGLNARGGLYLEULYSTHR
18   VALPHEASPGLUALAILEVALALAALALEU
19   GLU

Entity 2, GNP 1 residues - C10 H17 N6 O13 P3 - 522.196 Da.

1   GNP

Samples:

sample_1: Cell division control protein 42, [U-15N; U-13C], 0.7 mM; 5p-guanosyl-imido-triphosphate 7 mM; sodium phosphate 50 mM; magnesium chloride 2 mM; sodium chloride 50 mM

cond_1: pH*: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 4770
EMBL CAX45095 CCE43086 CCG21049
GB AAB69764 EAK92670 EAK92699 EDK42478 EEQ42372
REF XP_001528136 XP_002417442 XP_002548861 XP_003866489 XP_007376934
SP C4YDI6 P0CY33