BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4791

Title: Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase

Authors: Liu, Dingjiang; Wyss, Daniel

Citation: Liu, Dingjiang; Wyss, Daniel. "Letter to the Editor: Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase"  J. Biomol. NMR 18, 279-280 (2000).

Assembly members:
HCV helicase, polymer, 142 residues, Formula weight is not available

Natural source:   Common Name: hepatitis C virus   Taxonomy ID: 11103   Superkingdom: viruses   Kingdom: not available   Genus/species: hepatitis C virus hepatitis C virus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HCV helicase: GSHMGSVTVPHPNIEEVALS TTGEIPFYGKAIPLEVIKGG RHLIFCHSKKKCDELAAKLV ALGINAVAYYRGLDVSVIPT NGDVVVVATDALMTGFTGDF DSVIDCNTSDGKPQDAVSRT QRRGRTGRGKPGIYRFVAPG ER

Data sets:
Data typeCount
13C chemical shifts577
15N chemical shifts143
1H chemical shifts942

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV NS3 RNA helicase domain 21

Entities:

Entity 1, HCV NS3 RNA helicase domain 2 142 residues - Formula weight is not available

1   GLYSERHISMETGLYSERVALTHRVALPRO
2   HISPROASNILEGLUGLUVALALALEUSER
3   THRTHRGLYGLUILEPROPHETYRGLYLYS
4   ALAILEPROLEUGLUVALILELYSGLYGLY
5   ARGHISLEUILEPHECYSHISSERLYSLYS
6   LYSCYSASPGLULEUALAALALYSLEUVAL
7   ALALEUGLYILEASNALAVALALATYRTYR
8   ARGGLYLEUASPVALSERVALILEPROTHR
9   ASNGLYASPVALVALVALVALALATHRASP
10   ALALEUMETTHRGLYPHETHRGLYASPPHE
11   ASPSERVALILEASPCYSASNTHRSERASP
12   GLYLYSPROGLNASPALAVALSERARGTHR
13   GLNARGARGGLYARGTHRGLYARGGLYLYS
14   PROGLYILETYRARGPHEVALALAPROGLY
15   GLUARG

Samples:

sample-1: HCV helicase, [U-15N; U-13C], 0.2 – 1.0 mM; KPO4 75 mM; DTT, [U-2H], 5 mM; D2O 99%; AEBSF 0.4 mM; NaN3 0.015%

sample-2: HCV helicase, [U-15N; U-13C], 0.2 – 1.0 mM; KPO4 75 mM; DTT, [U-2H], 5 mM; D2O 5%; AEBSF 0.4 mM; NaN3 0.015%

cond_set_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
(H)CC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N-edited TOCSY-HSQCnot availablenot availablenot available

Software:

FELIX v98.0 - data processing

NMRView v4.0.3 - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAU44826 AAU44828 AAU44832 AAU44836 AAU44841