BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4792

Title: Backbone NMR Assignment and Secondary Structure of the Dimeric ParD Protein   PubMed: 11743881

Authors: Oberer, Monika; Prytulla, Stefan; Keller, Walter

Citation: Oberer, Monika; Zangger, Klaus; Prytulla, Stefan; Keller, Walter. "The Anti-toxin ParD of Plasmid RK2 Consists of two Structurally Distinct Moieties and Belongs to the Ribbon-helix-helix Family of DNA-binding Proteins"  Biochem. J. 361, 41-47 (2002).

Assembly members:
ParD, polymer, 83 residues, 9103.18 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ParD: MSRLTIDMTDQQHQSLKALA ALQGKTIKQYALERLFPGDA DADQAWQELKTMLGNRINDG LAGKVSTKSVGEILDEELSG DRA

Data sets:
Data typeCount
1H chemical shifts446
13C chemical shifts321
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ParD subunit 11
2ParD subunit 21

Entities:

Entity 1, ParD subunit 1 83 residues - 9103.18 Da.

1   METSERARGLEUTHRILEASPMETTHRASP
2   GLNGLNHISGLNSERLEULYSALALEUALA
3   ALALEUGLNGLYLYSTHRILELYSGLNTYR
4   ALALEUGLUARGLEUPHEPROGLYASPALA
5   ASPALAASPGLNALATRPGLNGLULEULYS
6   THRMETLEUGLYASNARGILEASNASPGLY
7   LEUALAGLYLYSVALSERTHRLYSSERVAL
8   GLYGLUILELEUASPGLUGLULEUSERGLY
9   ASPARGALA

Samples:

sample_1: ParD, [U-98% 13C; U-98% 15N], 0.6 mM

sample_2: ParD, [U-98% 15N], 0.5 mM

sample_3: ParD 0.5 mM

Cond1: pH: 6.0; temperature: 306 K; ionic strength: 0.11 M

Experiments:

NameSampleSample stateSample conditions
2D 1H15N HSQCnot availablenot availableCond1
2D NOESYnot availablenot availableCond1
2D TOCSYnot availablenot availableCond1
3D 15N NOESY-HSQCnot availablenot availableCond1
3D 15N TOCSY-HSQCnot availablenot availableCond1
3D HNCOnot availablenot availableCond1
3D HN(CO)CAnot availablenot availableCond1
3D CBCA(CO)NHnot availablenot availableCond1
3D HNCACBnot availablenot availableCond1

Software:

XEASY v1.3 -

NMR spectrometers:

  • Varian Unity INOVA 600 MHz

Related Database Links:

PDB
EMBL CAE54490 CAG30890 CAK12703
GB AAA26418 AAA91498 AAA92774 AAA98334 AAB67690
PIR A47048
REF WP_011205807 WP_032072691 YP_006941366 YP_006941448 YP_006941534
SP P22995
TPE CAJ85710