BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4797

Title: Sequence-specific resonance assignments of the potent cytolysin equinatoxin II

Authors: Zhang, Wei; Hinds, Mark; Anderluh, Gregor; Hansen, Poul; Norton, Raymond

Citation: Zhang, Wei; Hinds, Mark; Anderluh, Gregor; Hansen, Poul; Norton, Raymond. "Letter to the Editor: Sequence-specific resonance assignments of the potent cytolysin equinatoxin II"  J. Biomol. NMR 18, 281-282 (2000).

Assembly members:
Equinatoxin II, polymer, 179 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 6106   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Actinia equina

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Equinatoxin II: SADVAGAVIDGASLSFDILK TVLEALGNVKRKIAVGVDNE SGKTWTALNTYFRSGTSDIV LPHKVPHGKALLYNGQKDRG PVATGAVGVLAYLMSDGNTL AVLFSVPYDYNWYSNWWNVR IYKGKRRADQRMYEELYYNL SPFRGDNGWHTRNLGYGLKS RGFMNSSGHAILEIHVTKA

Data sets:
Data typeCount
1H chemical shifts1174
13C chemical shifts681
15N chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EqtII1

Entities:

Entity 1, EqtII 179 residues - Formula weight is not available

1   SERALAASPVALALAGLYALAVALILEASP
2   GLYALASERLEUSERPHEASPILELEULYS
3   THRVALLEUGLUALALEUGLYASNVALLYS
4   ARGLYSILEALAVALGLYVALASPASNGLU
5   SERGLYLYSTHRTRPTHRALALEUASNTHR
6   TYRPHEARGSERGLYTHRSERASPILEVAL
7   LEUPROHISLYSVALPROHISGLYLYSALA
8   LEULEUTYRASNGLYGLNLYSASPARGGLY
9   PROVALALATHRGLYALAVALGLYVALLEU
10   ALATYRLEUMETSERASPGLYASNTHRLEU
11   ALAVALLEUPHESERVALPROTYRASPTYR
12   ASNTRPTYRSERASNTRPTRPASNVALARG
13   ILETYRLYSGLYLYSARGARGALAASPGLN
14   ARGMETTYRGLUGLULEUTYRTYRASNLEU
15   SERPROPHEARGGLYASPASNGLYTRPHIS
16   THRARGASNLEUGLYTYRGLYLEULYSSER
17   ARGGLYPHEMETASNSERSERGLYHISALA
18   ILELEUGLUILEHISVALTHRLYSALA

Samples:

sample_1: Equinatoxin II, [U-95% 13C; U-95% 15N], 1.0 mM

sample_2: Equinatoxin II, [U-95% 15N], 1.0 mM

cond_set_1: pH: 3.9; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

XEASY -

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAB31198 AAC47005
PRF 2210238A
SP P61914 P61915