BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4798

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Frq1

Authors: Ames, James; Hendricks, Kristin; Strahl, Thomas; Huttner, Inken; Hamasaki, Nobuko; Thorner, Jeremy

Citation: Ames, James; Hendricks, Kristin; Strahl, Thomas; Huttner, Inken; Hamasaki, Nobuko; Thorner, Jeremy. "Structure and Calcium-binding Properties of frq1, a Novel Calcium Sensor in the Yeast Saccharomyces cerevisiae"  Biochemistry 39, 12149-12161 (2000).

Assembly members:
Yeast frequenin, polymer, 190 residues, 22000 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: budding yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Yeast frequenin: MGAKTSKLSKDDLTCLKQST YFDRREIQQWHKGFLRDCPS GQLAREDFVKIYKQFFPFGS PEDFANHLFTVFDKDNNGFI HFEEFITVLSTTSRGTLEEK LSWAFELYDLNHDGYITFDE MLTIVASVYKMMGSMVTLNE DEATPEMRVKKIFKLMDKNE DGYITLDEFREGSKVDPSII GALNLYDGLI

Data sets:
Data typeCount
1H chemical shifts393
13C chemical shifts355
15N chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Frq11
2Ca I2
3Ca II2
4Ca III2

Entities:

Entity 1, Frq1 190 residues - 22000 Da.

1   METGLYALALYSTHRSERLYSLEUSERLYS
2   ASPASPLEUTHRCYSLEULYSGLNSERTHR
3   TYRPHEASPARGARGGLUILEGLNGLNTRP
4   HISLYSGLYPHELEUARGASPCYSPROSER
5   GLYGLNLEUALAARGGLUASPPHEVALLYS
6   ILETYRLYSGLNPHEPHEPROPHEGLYSER
7   PROGLUASPPHEALAASNHISLEUPHETHR
8   VALPHEASPLYSASPASNASNGLYPHEILE
9   HISPHEGLUGLUPHEILETHRVALLEUSER
10   THRTHRSERARGGLYTHRLEUGLUGLULYS
11   LEUSERTRPALAPHEGLULEUTYRASPLEU
12   ASNHISASPGLYTYRILETHRPHEASPGLU
13   METLEUTHRILEVALALASERVALTYRLYS
14   METMETGLYSERMETVALTHRLEUASNGLU
15   ASPGLUALATHRPROGLUMETARGVALLYS
16   LYSILEPHELYSLEUMETASPLYSASNGLU
17   ASPGLYTYRILETHRLEUASPGLUPHEARG
18   GLUGLYSERLYSVALASPPROSERILEILE
19   GLYALALEUASNLEUTYRASPGLYLEUILE

Entity 2, Ca I - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Yeast frequenin, [U-95% 13C; U-90% 15N], 0.8 mM; octyl glucoside 20 mM

Ex-cond_1: pH: 6.6; temperature: 318 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond_1
1H-15N TOCSYsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCACONHsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ GAA22591
EMBL CAY78874
GB AAB64809 AHY75328 EDN60701 EDV07959 EDZ72897
REF NP_010661 XP_011104984
SP Q06389
TPG DAA12214