BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4825

Title: 1H, 15N and 13C NMR Resonance Assignments of RC-RNase 2

Authors: Hsu, Chun-Hua; Chen, Lih-Woan; Liao, You-Di; Wu, Shih-Hsiung; Chen, Chinpan

Citation: Hsu, Chun-Hua; Chen, Lih-Woan; Liao, You-Di; Wu, Shih-Hsiung; Chen, Chinpan. "1H, 15N and 13C resonance assignments and secondary structure determination of the RC-RNase 2 from oocytes of bullfrog Rana catesbeiana"  J. Biomol. NMR 19, 87-88 (2001).

Assembly members:
rRC-RNase2[M(-1),Q1], polymer, 106 residues, 12346 Da.

Natural source:   Common Name: bullfrog   Taxonomy ID: 8400   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana catesbeiana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rRC-RNase2[M(-1),Q1]: MQNWETFQKKHLTDTRDVKC DAEMKKALFDCKQKNTFIYA RPGRVQALCKNIIVSKNVLS TDEFYLSDCNRIKLPCHYKL KKSSNTICITCENKLPVHFV AVEECP

Data sets:
Data typeCount
1H chemical shifts768
13C chemical shifts490
15N chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rRC-RNase2[M(-1),Q1]1

Entities:

Entity 1, rRC-RNase2[M(-1),Q1] 106 residues - 12346 Da.

1   METGLNASNTRPGLUTHRPHEGLNLYSLYS
2   HISLEUTHRASPTHRARGASPVALLYSCYS
3   ASPALAGLUMETLYSLYSALALEUPHEASP
4   CYSLYSGLNLYSASNTHRPHEILETYRALA
5   ARGPROGLYARGVALGLNALALEUCYSLYS
6   ASNILEILEVALSERLYSASNVALLEUSER
7   THRASPGLUPHETYRLEUSERASPCYSASN
8   ARGILELYSLEUPROCYSHISTYRLYSLEU
9   LYSLYSSERSERASNTHRILECYSILETHR
10   CYSGLUASNLYSLEUPROVALHISPHEVAL
11   ALAVALGLUGLUCYSPRO

Samples:

sample_1: rRC-RNase2[M(-1),Q1], [U-15N; U-13C], 1.5 mM

cond-sample_1: pH: 3.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available

Software:

XWINNMR - data processing

AURELIA - assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GenBank AAG31439