BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4833

Title: 1H, 13C, and 15N assignments for the Antifungal protein from Streptomyces tendae Tu901   PubMed: 11350173

Authors: Campos-Olivas, Ramon; Horr, Ingmar; Bormann, Christiane; Jung, Guenther; Gronenborn, Angela

Citation: Campos-Olivas, Ramon; Horr, Ingmar; Bormann, Christiane; Jung, Guenther; Gronenborn, Angela. "Solution Structure, Backbone Dynamics and Chitin Binding of the Anti-fungal Protein from Streptomyces tendae Tu901"  J. Mol. Biol. 308, 765-782 (2001).

Assembly members:
Antifungal protein from Streptomyces tendae Tu901, polymer, 87 residues, 9999.2 Da.

Natural source:   Common Name: not available   Taxonomy ID: 1932   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces tendae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Antifungal protein from Streptomyces tendae Tu901: MINRTDCNENSYLEIHNNEG RDTLCFANAGTMPVAIYGVN WVESGNNVVTLQFQRNLSDP RLETITLQKWGSWNPGHIHE ILSIRIY

Data sets:
Data typeCount
1H chemical shifts597
13C chemical shifts376
15N chemical shifts97
coupling constants68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AFP-1 monomer1

Entities:

Entity 1, AFP-1 monomer 87 residues - 9999.2 Da.

1   METILEASNARGTHRASPCYSASNGLUASN
2   SERTYRLEUGLUILEHISASNASNGLUGLY
3   ARGASPTHRLEUCYSPHEALAASNALAGLY
4   THRMETPROVALALAILETYRGLYVALASN
5   TRPVALGLUSERGLYASNASNVALVALTHR
6   LEUGLNPHEGLNARGASNLEUSERASPPRO
7   ARGLEUGLUTHRILETHRLEUGLNLYSTRP
8   GLYSERTRPASNPROGLYHISILEHISGLU
9   ILELEUSERILEARGILETYR

Samples:

sample_1: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-10% 13C], 0.5 – 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 8.3%; H2O 91.7%

sample_2: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-13C], 0.5 – 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 8.3%; H2O 91.7%

sample_3: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-10% 13C], 0.5 – 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 100%

sample_4: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-13C], 0.5 – 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 100%

conditions_1: pH: 8.0; temperature: 293 K; ionic strength: 0.32 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQC/HMQCnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D 15N-edited NOESYnot availablenot availablenot available
4D 13C,13C NOESYnot availablenot availablenot available

Software:

NMRView v4.1.1. -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

EMBL CAB63113
PDB