BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4838

Title: NMR Mapping and Secondary Structure Determination of the Major Acetylcholine Receptor alpha-Subunit Determinant Interacting with alpha-Bungarotoxin   PubMed: 11331011

Authors: Samson, Abraham; Chill, Jordan; Rodriguez, Erik; Scherf, Tali; Anglister, Jacob

Citation: Samson, Abraham; Chill, Jordan; Rodriguez, Erik; Scherf, Tali; Anglister, Jacob. "NMR Mapping and Secondary Structure Determination of the Major Acetylcholine Receptor alpha-Subunit Determinant Interacting with alpha-Bungarotoxin"  Biochemistry 40, 5464-5473 (2001).

Assembly members:
alpha-bungarotoxin, polymer, 74 residues, Formula weight is not available
acetylcholine receptor peptide, polymer, 25 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 8616   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bungarus multicinctus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
alpha-bungarotoxin: IVCHTTATSPISAVTCPPGE NLCYRKMWCDAFCSSRGKVV ELGCAATCPSKKPYEEVTCC STDKCNPHPKRQPG
acetylcholine receptor peptide: EERGWKHWVYYTCCPDTPYL DITEE

Data sets:
Data typeCount
1H chemical shifts477
coupling constants79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1a-bungarotoxin1
2Acetylcholine receptor peptide2

Entities:

Entity 1, a-bungarotoxin 74 residues - Formula weight is not available

1   ILEVALCYSHISTHRTHRALATHRSERPRO
2   ILESERALAVALTHRCYSPROPROGLYGLU
3   ASNLEUCYSTYRARGLYSMETTRPCYSASP
4   ALAPHECYSSERSERARGGLYLYSVALVAL
5   GLULEUGLYCYSALAALATHRCYSPROSER
6   LYSLYSPROTYRGLUGLUVALTHRCYSCYS
7   SERTHRASPLYSCYSASNPROHISPROLYS
8   ARGGLNPROGLY

Entity 2, Acetylcholine receptor peptide 25 residues - Formula weight is not available

1   GLUGLUARGGLYTRPLYSHISTRPVALTYR
2   TYRTHRCYSCYSPROASPTHRPROTYRLEU
3   ASPILETHRGLUGLU

Samples:

sample_1: alpha-bungarotoxin 2 mM; acetylcholine receptor peptide 2 mM

ex-cond1: pH: 4; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HOHAHAnot availablenot availablenot available
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

Xwinnmr -

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 5025 5025
PDB
GB AAA96704 AAA96705 AAR29364 AAR29368 ABS70800
PIR A39718 A39718
REF XP_003438387 XP_004553560 XP_005733803 XP_005922532 XP_006783580
SP P02710 P02711
SWISS-PROT P02710 P02711
GenBank ABS70800 AAR29364 AAR29368 AAA96704 AAA96705