BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4872

Title: Interhelical Ion Pairing in Coiled Coils: Solution Structure of a Heterodimeric Leucine Zipper and Determination of pKa Values of Glu Side Chains

Authors: Marti, D.; Jelesarov, I.; Bosshard, H.

Citation: Marti, D.; Jelesarov, I.; Bosshard, H.. "Interhelical Ion Pairing in Coiled Coils: Solution Structure of a Heterodimeric Leucine Zipper and Determination of pKa Values of Glu Side Chains"  Biochemistry 39, 12804-12818 (2000).

Assembly members:
LEUCINE ZIPPER CHAIN A, polymer, 33 residues, 3557 Da.
LEUCINE ZIPPER CHAIN B, polymer, 33 residues, 3576 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
LEUCINE ZIPPER CHAIN A: XEVAQLEKEVAQAEAENYQLE QEVAQLEHECGX
LEUCINE ZIPPER CHAIN B: XEVQALKKRVQALKARNYAA KQKVQALRHKCGX

Data sets:
Data typeCount
1H chemical shifts456

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACIDIC CHAIN1
2BASIC CHAIN2

Entities:

Entity 1, ACIDIC CHAIN 33 residues - 3557 Da.

1   ACEGLUVALALAGLNLEUGLULYSGLUVAL
2   ALAGLNALAGLUALAGLUASNTYRGLNLEU
3   GLUGLNGLUVALALAGLNLEUGLUHISGLU
4   CYSGLYNH2

Entity 2, BASIC CHAIN 33 residues - 3576 Da.

1   ACEGLUVALGLNALALEULYSLYSARGVAL
2   GLNALALEULYSALAARGASNTYRALAALA
3   LYSGLNLYSVALGLNALALEUARGHISLYS
4   CYSGLYNH2

Samples:

sample_1: LEUCINE ZIPPER CHAIN A 2.1 mM; LEUCINE ZIPPER CHAIN B 2.1 mM

sample_cond_1: ionic strength: 10 mM; pH: 5.65; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1

Software:

xwinnmr v2.6 - data collection

FELIX v98 - data processing

X-PLOR v3.851 - structure calculation

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB