BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4879

Title: Assignment of the 1H, 13C and 15N Signals of Sortase

Authors: Ilangovan, Udayar; Iwahara, Junji; Ton-That, Hung; Schneewind, Olaf; Clubb, Robert

Citation: Ilangovan, Udayar; Iwahara, Junji; Ton-That, Hung; Schneewind, Olaf; Clubb, Robert. "Letter to the Editor: Assignment of the 1H, 13C and 15N Signals of Sortase"  J. Biomol. NMR 19, 379-380 (2001).

Assembly members:
Staphyloccal Sortase, polymer, 148 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Staphyloccal Sortase: MQAKPQIPKDKSKVAGYIEI PDADIKEPVYPGPATPEQLN RGVSFAEENESLDDQNISIA GHTFIDRPNYQFTNLKAAKK GSMVYFKVGNETRKYKMTSI RDVKPTDVGVLDEQKGKDKQ LTLITCDDYNEKTGVWEKRK IFVATEVK

Data sets:
Data typeCount
13C chemical shifts642
15N chemical shifts147
1H chemical shifts966

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sortase1

Entities:

Entity 1, Sortase 148 residues - Formula weight is not available

1   METGLNALALYSPROGLNILEPROLYSASP
2   LYSSERLYSVALALAGLYTYRILEGLUILE
3   PROASPALAASPILELYSGLUPROVALTYR
4   PROGLYPROALATHRPROGLUGLNLEUASN
5   ARGGLYVALSERPHEALAGLUGLUASNGLU
6   SERLEUASPASPGLNASNILESERILEALA
7   GLYHISTHRPHEILEASPARGPROASNTYR
8   GLNPHETHRASNLEULYSALAALALYSLYS
9   GLYSERMETVALTYRPHELYSVALGLYASN
10   GLUTHRARGLYSTYRLYSMETTHRSERILE
11   ARGASPVALLYSPROTHRASPVALGLYVAL
12   LEUASPGLUGLNLYSGLYLYSASPLYSGLN
13   LEUTHRLEUILETHRCYSASPASPTYRASN
14   GLULYSTHRGLYVALTRPGLULYSARGLYS
15   ILEPHEVALALATHRGLUVALLYS

Samples:

Sample_1: Staphyloccal Sortase 2.5 mM; Tris 50 mM; NaCl 100 mM; CaCl2 10 mM; DTT 3 mM; D2O 7%

Cond-1: pH*: 6.2; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C CT-HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-13C-1H HCCH-COSYnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 13C-1H-1H NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D 1H-13C-1H HCC-TOCSY-CCH-E.COSYnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
4D 1H-15N-1H-13C NOESYnot availablenot availablenot available
4D 1H-13C-13C-1H NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 16270 19624 19826
PDB
DBJ BAB43619 BAB58690 BAB96313 BAF68698 BAF79395
EMBL CAG41587 CAG44229 CAI82090 CAQ50958 CBI50513
GB AAD48437 AAW37316 ABD22861 ABD31836 ABQ50328
REF NP_373052 NP_375640 NP_647265 WP_000759357 WP_000759358