BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4880

Title: 1H, 15N and 13C resonance assignments of the N-terminal region of calponin

Authors: Bramham, Janice; Smith, Brian; Uhrin, Dusan; Barlow, Paul; Winder, Steven

Citation: Bramham, Janice; Smith, Brian; Uhrin, Dusan; Barlow, Paul; Winder, Steven. "Letter to the Editor: 1H, 15N and 13C resonance assignments of the N-terminal region of calponin"  J. Biomol. NMR 19, 189-190 (2001).

Assembly members:
calponin CH domain, polymer, 108 residues, 12249.96 Da.

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calponin CH domain: MPQTERQLRVWIEGATGRRI GDNFMDGLKDGVILCELINK LQPGSVQKVNDPVQNWHKLE NIGNFLRAIKHYGVKPHDIF EANDLFENTNHTQVQSTLIA LASQAKTK

Data sets:
Data typeCount
1H chemical shifts795
13C chemical shifts497
15N chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calponin CH domain1

Entities:

Entity 1, calponin CH domain 108 residues - 12249.96 Da.

1   METPROGLNTHRGLUARGGLNLEUARGVAL
2   TRPILEGLUGLYALATHRGLYARGARGILE
3   GLYASPASNPHEMETASPGLYLEULYSASP
4   GLYVALILELEUCYSGLULEUILEASNLYS
5   LEUGLNPROGLYSERVALGLNLYSVALASN
6   ASPPROVALGLNASNTRPHISLYSLEUGLU
7   ASNILEGLYASNPHELEUARGALAILELYS
8   HISTYRGLYVALLYSPROHISASPILEPHE
9   GLUALAASNASPLEUPHEGLUASNTHRASN
10   HISTHRGLNVALGLNSERTHRLEUILEALA
11   LEUALASERGLNALALYSTHRLYS

Samples:

sample_1: calponin CH domain, [U-99% 15N; U-98% 15N], 1.0 mM

conditions_1: pH*: 7.0; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
1H,15N-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CBCA)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
H(C)(CO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available
(HB)CB(CGCDCE)HEnot availablenot availablenot available
13C,15N HSQC-NOESY-CH3NHnot availablenot availablenot available

Software:

AZARA - Processing raw data

ANSIG - peak assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA48651 AAA48652 AAB30248 ABD17727 ABD17728
PRF 1702210A
REF NP_990847 XP_010725501
SP P26932 P37803