BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4884

Title: 1st LIM domain of PINCH protein   PubMed: 11078733

Authors: Velyvis, Algirdas; Yang, Yanwu; Wu, Chuanyue; Qin, Jun

Citation: Velyvis, Algirdas; Yang, Yanwu; Wu, Chuanyue; Qin, Jun. "Solution Structure of Focal Adhesion Adaptor PINCH LIM1 Domain and Characterization of Its Interaction with Integrin Linked Kinase Ankyrin Repeat Domain"  J. Biol. Chem. 276, 4932-4939 (2001).

Assembly members:
1st LIM domain of PINCH protein, polymer, 77 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
1st LIM domain of PINCH protein: ISEFMANALASATCERCKGG FAPAEKIVNSNGELYHEQCF VCAQCFQQFPEGLFYEFEGR KYCEHDFQMLFAPCWIL

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts67
1H chemical shifts410
13C chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LIM1 domain1
2Zn-12
3Zn-22

Entities:

Entity 1, LIM1 domain 77 residues - Formula weight is not available

1   ILESERGLUPHEMETALAASNALALEUALA
2   SERALATHRCYSGLUARGCYSLYSGLYGLY
3   PHEALAPROALAGLULYSILEVALASNSER
4   ASNGLYGLULEUTYRHISGLUGLNCYSPHE
5   VALCYSALAGLNCYSPHEGLNGLNPHEPRO
6   GLUGLYLEUPHETYRGLUPHEGLUGLYARG
7   LYSTYRCYSGLUHISASPPHEGLNMETLEU
8   PHEALAPROCYSTRPILELEU

Entity 2, Zn-1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: 1st LIM domain of PINCH protein 0.5 mM; Na phosphate 50 mM; NaCl 100 mM; beta-mercaptoethanol 0.5 mM

sample_2: 1st LIM domain of PINCH protein, [U-15N], 0.5 mM; Na phosphate 50 mM; NaCl 100 mM; beta-mercaptoethanol 0.5 mM

sample_3: 1st LIM domain of PINCH protein, [U-15N; U-13C], 0.5 mM; Na phosphate 50 mM; NaCl 100 mM; beta-mercaptoethanol 0.5 mM

representative_conditions: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablerepresentative_conditions
3D CBCANHnot availablenot availablerepresentative_conditions
3D CBCA(CO)NHnot availablenot availablerepresentative_conditions
3D 1H-1H-13C/15N NOESYnot availablenot availablerepresentative_conditions
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablerepresentative_conditions
3D C(CO)NHnot availablenot availablerepresentative_conditions
3D H(CCO)NHnot availablenot availablerepresentative_conditions
3D HNHAnot availablenot availablerepresentative_conditions
2D 1H-1H NOESYnot availablenot availablerepresentative_conditions
2D 1H-1H TOCSYnot availablenot availablerepresentative_conditions

Software:

nmrPipe -

PIPP -

NMR spectrometers:

  • Varian Unity Plus 500 MHz

Related Database Links:

SWISS-PROT Q9HB10
REF XP_862963 NP_277049
GenBank AAY24131 AAY14903 AAI12234 AAH93812 AAF99328
DBJ BAG60588 BAG35111
PDB