BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4890

Title: 1H resonance assignments for the N-ter domain of the human TFIIH MAT1 subunit

Authors: Gervais, V.; Wasielewski, E.; Busso, D.; Poterszman, A.; Egly, J.M.; Thierry, J.C.; Kieffer, B.

Citation: Gervais, V.; Busso, D.; Wasielewski, E.; Poterszman, A.; Egly, J.M.; Thierry, J.C.; Kieffer, B.. "Solution Structure of the N-terminal Domain of the Human TFIIH MAT1 Subunit: New Insights into the RING Finger Family "  J. Biol. Chem. ., .-..

Assembly members:
Menage a trois, polymer, 65 residues, 7296 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Menage a trois: MDDQGCPRCKTTKYRNPSLK LMVNVCGHTLCESCVDLLFV RGAGNCPECGTPLRKSNFRV QLFED

Data sets:
Data typeCount
1H chemical shifts424

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAT11
2Zn(II)-12
3Zn(II)-22

Entities:

Entity 1, MAT1 65 residues - 7296 Da.

1   METASPASPGLNGLYCYSPROARGCYSLYS
2   THRTHRLYSTYRARGASNPROSERLEULYS
3   LEUMETVALASNVALCYSGLYHISTHRLEU
4   CYSGLUSERCYSVALASPLEULEUPHEVAL
5   ARGGLYALAGLYASNCYSPROGLUCYSGLY
6   THRPROLEUARGLYSSERASNPHEARGVAL
7   GLNLEUPHEGLUASP

Entity 2, Zn(II)-1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Menage a trois 2 mM

MAT1_conditions: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availableMAT1_conditions
DQF-COSYsample_1not availableMAT1_conditions
2D TOCSYsample_1not availableMAT1_conditions

Software:

X-PLOR v3.8 - structure solution, refinement

XWINNMR v1.3 - data collection, data processing

xeasy v1.3.13 - data analysis

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

SWISS-PROT P51948 P51949
REF NP_703202 XP_001096910 NP_002422 NP_032638 NP_001098879
GenBank AAH89023 AAH00820 AAH78712 AAA91741 AAB05248
EMBL CAA63356 CAG29332 CAA61112
DBJ BAE34168 BAB25556 BAE33464 BAB25483
PDB