BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4892

Title: 1H, 13C, and 15N Chemical Shift Assignments for apo-Mts1 (S100A4)

Authors: Rustandi, Richard; Vallely, Kristen; Varlamova, Olga; Klein, Michael; Almo, Steven; Bresnick, Anne; Weber, David

Citation: Rustandi, Richard; Vallely, Kristen; Varlamova, Olga; Klein, Michael; Almo, Steven; Bresnick, Anne; Weber, David. "Letter to the Editor: 1H, 13C and 15N NMR sequence-specific resonance assignments for human apo-Mts1 (S100A4)"  J. Biomol. NMR 19, 381-382 (2001).

Assembly members:
S100A4, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
S100A4: MACPLEKALDVMVSTFHKYS GKEGDKFKLNKSELKELLTR ELPSFLGKRTDEAAFQKLMS NLDSNRDNEVDFQEYCVFLS CIAMMCNEFFEGFPDKQPRK K

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts425
13C chemical shifts244
15N chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S100A4 subunit 11
2S100A4 subunit 21

Entities:

Entity 1, S100A4 subunit 1 101 residues - Formula weight is not available

1   METALACYSPROLEUGLULYSALALEUASP
2   VALMETVALSERTHRPHEHISLYSTYRSER
3   GLYLYSGLUGLYASPLYSPHELYSLEUASN
4   LYSSERGLULEULYSGLULEULEUTHRARG
5   GLULEUPROSERPHELEUGLYLYSARGTHR
6   ASPGLUALAALAPHEGLNLYSLEUMETSER
7   ASNLEUASPSERASNARGASPASNGLUVAL
8   ASPPHEGLNGLUTYRCYSVALPHELEUSER
9   CYSILEALAMETMETCYSASNGLUPHEPHE
10   GLUGLYPHEPROASPLYSGLNPROARGLYS
11   LYS

Samples:

sample_1: S100A4, [U-13C; U-15N], 3 mM

S100A4-cond_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H,15N-HSQCnot availablenot availablenot available
3D 15N-edited NOESY-HSQCnot availablenot availablenot available
3D 15N-edited HOHAHA-HSQCnot availablenot availablenot available
3D 15N,15N-edited HMQC-NOESY-HSQCnot availablenot availablenot available
3D 15N HNHAnot availablenot availablenot available
3D CCONHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
4D 13C,15N-edited NOESY-HSQCnot availablenot availablenot available
4D 13C,13C-edited NOESY-HSQCnot availablenot availablenot available

Software:

nmrPipe -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 18169
PDB
DBJ BAF84772 BAG74210
EMBL CAA79474 CAA83880 CAG29341
GB AAA51920 AAH00838 AAH16300 AAV74399 AAX29777
REF NP_001239534 NP_002952 NP_062427 XP_001110635 XP_001110673
SP P26447