BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4893

Title: 1H, 15N and 13C NMR Resonance Assignments of RC-RNase 4   PubMed: 11430762

Authors: Hsu, Chun-Hua; Liao, You-Di; Wu, Shih-Hsiung; Chen, Chinpan

Citation: Hsu, Chun-Hua; Liao, You-Di; Wu, Shih-Hsiung; Chen, Chinpan. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments and Secondary Structure of the Cytotoxic Protein RNase 4 from Bullfrog Rana catesbeiana Oocytes"  J. Biomol. NMR 20, 93-94 (2001).

Assembly members:
rRC-RNase4[M(-1),Q1], polymer, 107 residues, 12217 Da.

Natural source:   Common Name: bullfrog   Taxonomy ID: 8400   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana catesbeiana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rRC-RNase4[M(-1),Q1]: MQDWATFKKKHLTDTWDVDC DNLMPTSLFDCKDKNTFIYS LPGPVKALCRGVIFSADVLS NSEFYLAECNVKPRKPCKYK LKKSSNRICIRCEHELPVHF AGVGICP

Data sets:
Data typeCount
1H chemical shifts766
13C chemical shifts494
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rRC-RNase41

Entities:

Entity 1, rRC-RNase4 107 residues - 12217 Da.

1   METGLNASPTRPALATHRPHELYSLYSLYS
2   HISLEUTHRASPTHRTRPASPVALASPCYS
3   ASPASNLEUMETPROTHRSERLEUPHEASP
4   CYSLYSASPLYSASNTHRPHEILETYRSER
5   LEUPROGLYPROVALLYSALALEUCYSARG
6   GLYVALILEPHESERALAASPVALLEUSER
7   ASNSERGLUPHETYRLEUALAGLUCYSASN
8   VALLYSPROARGLYSPROCYSLYSTYRLYS
9   LEULYSLYSSERSERASNARGILECYSILE
10   ARGCYSGLUHISGLULEUPROVALHISPHE
11   ALAGLYVALGLYILECYSPRO

Samples:

sample_1: rRC-RNase4[M(-1),Q1], [U-15N; U-13C], 1.5 mM

cond-sample_1: pH: 3.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available

Software:

XWINNMR - data processing

AURELIA - assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAG31441