BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4900

Title: 1H and 15N chemical shift assignments of the C-terminal xylan binding module of C. fimi xylanase 11A   PubMed: 11327868

Authors: Bolam, David; Xie, Hefang; White, Peter; Simpson, Peter; Hancock, Susan; Williamson, Michael; Gilbert, Harry

Citation: Bolam, David; Xie, Hefang; White, Peter; Simpson, Peter; Hancock, Susan; Williamson, Michael; Gilbert, Harry. "Evidence for Synergy Between Family 2b Carbohydrate Binding Modules in Cellulomonas fimi xylanase 11A"  Biochemistry 40, 2468-2477 (2001).

Assembly members:
xylanase 11A, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1708   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Cellulomonas fimi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
xylanase 11A: TGSCSVSAVRGEEWADRFNV TYSVSGSSSWVVTLGLNGGQ SVQSSWNAALTGSSGTVTAR PNGSGNSFGVTFYKNGSSAT PGATCATG

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts97
1H chemical shifts525

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1XBD1

Entities:

Entity 1, XBD 88 residues - Formula weight is not available

1   THRGLYSERCYSSERVALSERALAVALARG
2   GLYGLUGLUTRPALAASPARGPHEASNVAL
3   THRTYRSERVALSERGLYSERSERSERTRP
4   VALVALTHRLEUGLYLEUASNGLYGLYGLN
5   SERVALGLNSERSERTRPASNALAALALEU
6   THRGLYSERSERGLYTHRVALTHRALAARG
7   PROASNGLYSERGLYASNSERPHEGLYVAL
8   THRPHETYRLYSASNGLYSERSERALATHR
9   PROGLYALATHRCYSALATHRGLY

Samples:

sample_1: xylanase 11A 1.0 mM

sample_2: xylanase 11A, [U-99% 15N], 1.0 mM

Ex-cond_1: pH: 5.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
E.COSYnot availablenot availablenot available

Software:

FELIX v97 -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

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