BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4902

Title: Complete sequence-specific 1H, 13C and 15N resonance assignments of a novel hPTK6 SH2 domain

Authors: Hong, Eunmi; Shin, Joon; Lee, Weontae

Citation: Hong, Eunmi; Shin, Joon; Bang, Eunjung; Kim, Min-Hyung; Lee, Seung-Taek; Lee, Weontae. "Letter to the Editor: Complete sequence-specific 1H, 13C and 15N resonance assignments of the human PTK6 SH2 domain"  J. Biomol. NMR 19, 291-292 (2001).

Assembly members:
SH2 domian of Human Protein Tyrosine Kinase6, polymer, 100 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SH2 domian of Human Protein Tyrosine Kinase6: SEPWFFGCISRSEAVRRLQA EGNATGAFLIRVSEKPSADY VLSVRDTQAVRHYKIWRRAG GRLHLNEAVSFLSLPELVNY HRAQSLSHGLRLAAPCRKHE

Data sets:
Data typeCount
1H chemical shifts661
13C chemical shifts403
15N chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2 domain1

Entities:

Entity 1, SH2 domain 100 residues - Formula weight is not available

1   SERGLUPROTRPPHEPHEGLYCYSILESER
2   ARGSERGLUALAVALARGARGLEUGLNALA
3   GLUGLYASNALATHRGLYALAPHELEUILE
4   ARGVALSERGLULYSPROSERALAASPTYR
5   VALLEUSERVALARGASPTHRGLNALAVAL
6   ARGHISTYRLYSILETRPARGARGALAGLY
7   GLYARGLEUHISLEUASNGLUALAVALSER
8   PHELEUSERLEUPROGLULEUVALASNTYR
9   HISARGALAGLNSERLEUSERHISGLYLEU
10   ARGLEUALAALAPROCYSARGLYSHISGLU

Samples:

sample-1: SH2 domian of Human Protein Tyrosine Kinase6, [U-98% 13C; U-99.8% 15N], 0.5 – 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 298 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HMQC-Jnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCACOnot availablenot availablenot available
HNCACOnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

nmrPipe - data processing

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAG37660 BAI45737
EMBL CAA55295
GB AAC34935 AAH35843 EAW75262 EAW75263
REF NP_005966 XP_002830575 XP_003280201 XP_003809959 XP_004062576
SP Q13882