BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4910

Title: 1H and 15N chemical shift assignments for LEKTI domain one (HF6478)   PubMed: 12684009

Authors: Lauber, Thomas; Adermann, Knut; Schulz, Axel; Forssmann, Wolf; Roesch, Paul; Marx, Ute

Citation: Lauber, Thomas; Schulz, Axel; Schweimer, K.; Adermann, Knut; Marx, Ute. "Homologous proteins with different folds: the three-dimensional structures of domains 1 and 6 of the multiple Kazal-type inhibitor LEKTI"  J. Mol. Biol. 328, 205-219 (2003).

Assembly members:
HF6478, polymer, 55 residues, 6478 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HF6478: KNEDQEMCHEFQAFMKNGKL FCPQDKKFFQSLDGIMFINK CATCKMILEKEAKSQ

Data sets:
Data typeCount
15N chemical shifts51
1H chemical shifts686
coupling constants13

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HF64781

Entities:

Entity 1, HF6478 55 residues - 6478 Da.

1   LYSASNGLUASPGLNGLUMETCYSHISGLU
2   PHEGLNALAPHEMETLYSASNGLYLYSLEU
3   PHECYSPROGLNASPLYSLYSPHEPHEGLN
4   SERLEUASPGLYILEMETPHEILEASNLYS
5   CYSALATHRCYSLYSMETILELEUGLULYS
6   GLUALALYSSERGLN

Samples:

sample_1: HF6478, [U-15N], 1.5 mM

sample_2: HF6478 1.2 mM

Ex-cond_1: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D-TOCSYnot availablenot availablenot available
2D-NOESYnot availablenot availablenot available
2D-COSYnot availablenot availablenot available
1H-15N-HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D-1H-15N-TOCSY-HSQCnot availablenot availablenot available
3D-1H-15N-NOESY-HSQCnot availablenot availablenot available
3D-1H-15N/1H-15N-HMQC-NOESY-HSQCnot availablenot availablenot available

Software:

NDEE, Spinup Inc. -

NMRView v4.1.0 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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