BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4911

Title: Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain   PubMed: 11178896

Authors: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie

Citation: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain"  J. Mol. Biol. 306, 97-108 (2001).

Assembly members:
DYNEIN light chain 8, polymer, 89 residues, 10300 Da.
nNOS peptide, polymer, 18 residues, 2000 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: E. coli

Entity Sequences (FASTA):
DYNEIN light chain 8: MCDRKAVIKNADMSEEMQQD SVECATQALEKYNIEKDIAA HIKKEFDKKYNPTWHCIVGR NFGSYVTHETKHFIYFYLGQ VAILLFKSG
nNOS peptide: MKDTKIQVDRDLDGKSHK

Data sets:
Data typeCount
1H chemical shifts632
13C chemical shifts259
15N chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DYNEIN light chain 8, DLC8, subunit a1
2DYNEIN light chain 8, DLC8, subunit b1
3nNOS fragment peptide, subunit a2
4nNOS fragment peptide, subunit b2

Entities:

Entity 1, DYNEIN light chain 8, DLC8, subunit a 89 residues - 10300 Da.

1   METCYSASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   SERVALGLUCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   HISILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   LYSHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Entity 2, nNOS fragment peptide, subunit a 18 residues - 2000 Da.

1   METLYSASPTHRLYSILEGLNVALASPARG
2   ASPLEUASPGLYLYSSERHISLYS

Samples:

sample_1: DYNEIN light chain 8, [U-13C; U-15N], 1.5 mM; nNOS peptide, [U-13C; U-15N], 1.5 mM; phosphate buffer 100 mM; DTT 10 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.0; temperature: 303 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCACB and CBCACONNH for backbone assignmentsample_1not availablesample_cond_1
HCCH-TOCSY for side-chain assignmentsample_1not availablesample_cond_1

Software:

VNMR v6.1b - data collection

NMRPipe v1.7 - processing

X-PLOR v3.8 - structure solution

CNS v1.0 - spectra, refinement

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

SWISS-PROT P63169 P63168 P63167 P61285 P61273
REF NP_003737 NP_001075487 NP_001032584 NP_001032583 NP_001003901
GenBank AAD01643 AAC32531 AAC32530 AAB38257 AAB04149
EMBL CAG46925 CAG28600
DBJ BAB28973 BAB28970 BAB27117 BAB27063 BAB22160
PDB
BMRB 4931 4912 4305