BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4912

Title: Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain   PubMed: 11178896

Authors: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie

Citation: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain"  J. Mol. Biol. 306, 97-108 (2001).

Assembly members:
dynein light chain 8, polymer, 89 residues, 10300 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
dynein light chain 8: MCDRKAVIKNADMSEEMQQD SVECATQALEKYNIEKDIAA HIKKEFDKKYNPTWHCIVGR NFGSYVTHETKHFIYFYLGQ VAILLFKSG

Data sets:
Data typeCount
1H chemical shifts616
13C chemical shifts248
15N chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DYNEIN light chain 8, DLC8, subunit a1
2DYNEIN light chain 8, DLC8, subunit b1

Entities:

Entity 1, DYNEIN light chain 8, DLC8, subunit a 89 residues - 10300 Da.

1   METCYSASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   SERVALGLUCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   HISILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   LYSHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Samples:

sample_1: dynein light chain 8, [U-13C;U-15N], 1.5 mM; phosphate buffer 100 mM; DTT 10 mM; D2O 99.99%

sample_2: dynein light chain 8, [U-15N], 1.5 mM; phosphate buffer 100 mM; DTT 10 mM; D2O 10%; H2O 90%

sample_3: dynein light chain 8, [U-15N; U-13C], 1.5 mM; phosphate buffer 100 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

VNMR v6.1b - data collection

NMRPipe v1.7 - data processing

X-PLOR v3.8 - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

BMRB 4305
PDB
DBJ BAB22160 BAB27063 BAB27117 BAB28970 BAB28973
EMBL CAG28600 CAG46925 CAH65122 CAZ64801 CAZ64802
GB AAB04149 AAB38257 AAC32530 AAC32531 AAD01643
REF NP_001003901 NP_001032583 NP_001032584 NP_001075487 NP_001182043
SP P61273 P61285 P63167 P63168 P63169
TPG DAA17449 DAA20577 DAA23836