BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4915

Title: Structure and Function of the C-terminal PABC Domain of Human Poly(A)-binding Protein   PubMed: 11287632

Authors: Kozlov, G.; Trempe, J.-F.; Khaleghpour, K.; Kahvejian, A.; Ekiel, I.; Gehring, K.

Citation: Kozlov, G.; Trempe, J.-F.; Khaleghpour, K.; Kahvejian, A.; Ekiel, I.; Gehring, K.. "Structure and Function of the C-terminal PABC Domain of Human Poly(A)-binding Protein"  Proc. Natl. Acad. Sci. U.S.A. 98, 4409-4413 (2001).

Assembly members:
C-terminal domain of PABP, polymer, 144 residues, 15271 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
C-terminal domain of PABP: GPLGSAAAATPAVRTVPQYK YAAGVRNPQQHLNAQPQVTM QQPAVHVQGQEPLTASMLAS APPQEQKQMLGERLFPLIQA MHPTLAGKITGMLLEIDNSE LLHMLESPESLRSKVDEAVA VLQAHQAKEAAQKAVNSATG VPTV

Data sets:
Data typeCount
1H chemical shifts833
13C chemical shifts504
15N chemical shifts126
coupling constants101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Polyadenylate-binding protein 11

Entities:

Entity 1, Polyadenylate-binding protein 1 144 residues - 15271 Da.

1   GLYPROLEUGLYSERALAALAALAALATHR
2   PROALAVALARGTHRVALPROGLNTYRLYS
3   TYRALAALAGLYVALARGASNPROGLNGLN
4   HISLEUASNALAGLNPROGLNVALTHRMET
5   GLNGLNPROALAVALHISVALGLNGLYGLN
6   GLUPROLEUTHRALASERMETLEUALASER
7   ALAPROPROGLNGLUGLNLYSGLNMETLEU
8   GLYGLUARGLEUPHEPROLEUILEGLNALA
9   METHISPROTHRLEUALAGLYLYSILETHR
10   GLYMETLEULEUGLUILEASPASNSERGLU
11   LEULEUHISMETLEUGLUSERPROGLUSER
12   LEUARGSERLYSVALASPGLUALAVALALA
13   VALLEUGLNALAHISGLNALALYSGLUALA
14   ALAGLNLYSALAVALASNSERALATHRGLY
15   VALPROTHRVAL

Samples:

sample_1: C-terminal domain of PABP, [U-15N], 2.5 – 3.0 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_2: C-terminal domain of PABP, [U-15N; U-13C], 3.0 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; D2O 100%

sample_3: C-terminal domain of PABP 3.0 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.3; temperature: 303 K; ionic strength: 0.2 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

XWINNMR v2.1 - data collection

GIFA v4.31 - data processing

XEASY v1.3.13 - data analysis

CNS v0.9 - structure solution

ARIA v0.9 - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian UnityPlus 800 MHz

Related Database Links:

BMRB 10019 7033
PDB
DBJ BAC32110 BAC40951 BAC56450 BAE21553 BAE30919
EMBL CAA46522 CAA68428 CAB96752 CAH91893 CAH91953
GB AAD08718 AAH03870 AAH11207 AAH15958 AAH23145
REF NP_001126097 NP_001128842 NP_001244826 NP_002559 NP_032800
SP P11940 P29341 P61286 Q5R8F7
TPG DAA22579