BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4916

Title: RECEPTOR-BOUND CONFORMATION OF PACAP21   PubMed: 11175907

Authors: Inooka, H.; Ohtaki, T.; Kitahara, O.; Ikegami, T.; Endo, S.; Kitada, C.; Ogi, K.; Onda, H.; Fujino, M.; Shirakawa, M.

Citation: Inooka, H.; Ohtaki, T.; Kitahara, O.; Ikegami, T.; Endo, S.; Kitada, C.; Ogi, K.; Onda, H.; Fujino, M.; Shirakawa, M.. "Conformation of a Peptide Ligand bound to its G-protein Coupled Receptor"  Nat. Struct. Biol. 8, 161-165 (2001).

Assembly members:
Human peptide hormone, polymer, 22 residues, 2516.7 Da.
G-protein coupled receptor (GPCR), polymer, . residues, 16.023 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Human peptide hormone: HSDGIFTDSYSRYRKQMAVK KX

Data sets:
Data typeCount
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Peptide hormone for a G-protein coupled receptor (GPCR)1
2G-protein coupled receptor (GPCR)2

Entities:

Entity 1, Peptide hormone for a G-protein coupled receptor (GPCR) 22 residues - 2516.7 Da.

1   HISSERASPGLYILEPHETHRASPSERTYR
2   SERARGTYRARGLYSGLNMETALAVALLYS
3   LYSNH2

Entity 2, G-protein coupled receptor (GPCR) - 16.023 Da.

Samples:

sample_1: Human peptide hormone 1.7 mM; G-protein coupled receptor (GPCR) mM

sample_2: Human peptide hormone mM; G-protein coupled receptor (GPCR) mM

sample_cond_1: pH: 6.3; temperature: 298 K; ionic strength: 0.41 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_cond_1

Software:

X-PLOR v3.1 - Simulated annealing

IRMA v30-Jul-90 - iterative matrix relaxation

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 11058 11059 15166
PDB
DBJ BAA28355 BAC21153 BAC21154 BAC21155 BAC21156
EMBL CAA42962 CAA51705 CAA55684 CAA56564 CAG10213
GB AAA31575 AAA41791 AAB20402 AAB21469 AAB21470
PRF 2107317A
REF NP_001001544 NP_001009776 NP_001040020 NP_001081947 NP_001093203
SP O70176 P0DJ95 P13589 P16613 P18509
TPG DAA15829