BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4917

Title: Solution structure of a lipid transfer protein extracted from rice seeds. Comparison with homologous proteins   PubMed: 10092854

Authors: Poznanski, Jarek

Citation: Poznanski, Jaroslaw; Sodano, Patric; Suh, Se; Lee, Jae; Ptak, Marius; Vovelle, Francoise. "Solution structure of a lipid transfer protein extracted from rice seeds. Comparison with homologous proteins"  Eur. J. Biochem. 259, 692-708 (1999).

Assembly members:
Nonspecific Lipid Transfer Protein, polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: Rice   Taxonomy ID: 4530   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oryza sativa

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Nonspecific Lipid Transfer Protein: ITCGQVNSAVGPCLTYARGG AGPSAACCSGVRSLKAAAST TADRRTACNCLKNAARGIKG LNAGNAASIPSKCGVSVPYT ISASIDCSRVS

Data sets:
Data typeCount
1H chemical shifts1110
coupling constants61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rice ns-LTP1

Entities:

Entity 1, rice ns-LTP 91 residues - Formula weight is not available

1   ILETHRCYSGLYGLNVALASNSERALAVAL
2   GLYPROCYSLEUTHRTYRALAARGGLYGLY
3   ALAGLYPROSERALAALACYSCYSSERGLY
4   VALARGSERLEULYSALAALAALASERTHR
5   THRALAASPARGARGTHRALACYSASNCYS
6   LEULYSASNALAALAARGGLYILELYSGLY
7   LEUASNALAGLYASNALAALASERILEPRO
8   SERLYSCYSGLYVALSERVALPROTYRTHR
9   ILESERALASERILEASPCYSSERARGVAL
10   SER

Samples:

sample_1: Nonspecific Lipid Transfer Protein 5 mM; phosphate buffer 50 mM

t25: pH*: 6.4; temperature: 298 K

t10: pH*: 6.4; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
COSYsample_1not availablenot available
TOCSYsample_1not availablenot available
NOESYsample_1not availablenot available
P-COSYsample_1not availablenot available
2Q-COSYsample_1not availablenot available

Software:

UXNMR v930601.3 - Processing

XEASY v1.3.11 - signal assignment

DIANA v1.? - simulated annealing in the redac strategy

X-PLOR v3.1 - final refinement

NMR spectrometers:

  • Bruker AMX 500 MHz

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