BMRB Entry 4922

Name: Backbone 1H, 13C and 15N chemical shift assignments for unfolded apoplastocyanin
Published: 2001-08-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4922

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N chemical shift assignments for unfolded apoplastocyanin

Deposition date:

2000-12-13

Original release date:

2001-08-08

Authors:

Bai, Yawen; Chung, John; Dyson, Jane; Wright, Peter

Citation:

Citation: Bai, Yawen; Chung, John; Dyson, H. Jane; Wright, Peter. "Structural and Dynamic Characterization of an Unfolded State of Poplar Apo-plastocyanin Formed under Non-denaturing Conditions" Protein Sci. 10, 1056-1066 (2001).
PubMed: 11316886

Assembly members:

Assembly members:
unfolded apo-plastocyanin, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source: Common Name: poplar Taxonomy ID: 3691 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus nigra

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
unfolded apo-plastocyanin: IDVLLGADDGSLAFVPSEFS ISPGEKIVFKNNAGFPHNIV FDEDSIPSGVDASKISMSEE DLLNAKGETFEVALSNKGEY SFYCSPHQGAGMVGKVTVN

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	304
13C chemical shifts	270
15N chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unfolded apo-Pc	1

Entities:

Entity 1, unfolded apo-Pc 99 residues - Formula weight is not available

1

ILE

ASP

VAL

LEU

GLY

ALA

ASP

GLY

2

SER

LEU

ALA

PHE

VAL

PRO

SER

GLU

PHE

SER

3

ILE

SER

PRO

GLY

GLU

LYS

ILE

VAL

PHE

LYS

4

ASN

ALA

GLY

PHE

PRO

HIS

ASN

ILE

VAL

5

PHE

ASP

GLU

ASP

SER

ILE

PRO

SER

GLY

VAL

6

ASP

ALA

SER

LYS

ILE

SER

MET

SER

GLU

7

ASP

LEU

ASN

ALA

LYS

GLY

GLU

THR

PHE

8

GLU

VAL

ALA

LEU

SER

ASN

LYS

GLY

GLU

TYR

9

SER

PHE

TYR

CYS

SER

PRO

HIS

GLN

GLY

ALA

10

GLY

MET

VAL

GLY

LYS

VAL

THR

VAL

ASN

Samples:

sample_1: unfolded apo-plastocyanin, [U-15N], 2.0 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

sample_2: unfolded apo-plastocyanin, [U-15N; U-13C], 1.5 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

sample_3: unfolded apo-plastocyanin, [U-15N; U-13C], 0.5 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

sample_4: unfolded apo-plastocyanin 0.1 mM; KH2PO4 5 mM; EDTA 50 uM; beta-mercaptoethanol 5 mM

Ex-cond_1: pH: 6.0; temperature: 308 K; ionic strength: 5 mM

Experiments:

Name	Sample	Sample state	Sample conditions
15N NOESY-HSQC	not available	not available	not available
C(CO)NH-TOCSY	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
TOCSY-HSQC	not available	not available	not available
HNCACB	not available	not available	not available
HNCA	not available	not available	not available
HNCO	not available	not available	not available
(HA)CO(CA)NH(particularly useful for unfolded proteins)	not available	not available	not available

Software:

FELIX - Fourier transformation, analysis

NMR spectrometers:

Bruker AMX 500 MHz

BMRB	19247 4019
PDB	1JXG 1PLC 1PNC 1PND 1TKW 2PCY 3PCY 4DP7 4DP8 4DP9 4DPA 4DPB 4DPC 4PCY 5PCY 6PCY
EMBL	CAA90564
GB	ABK96770 EEE94750
REF	XP_002307754 XP_011003094
SP	P00299
AlphaFold	P00299