BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4923

Title: 1H chemical shift assignments for omega-atracotoxin-Hv2a   PubMed: 11522785

Authors: Wang, Xiu-hong; King, Glenn

Citation: Wang, Xiu-hong; Connor, M.; Wilson, D.; Wilson, H.; Nicholson, G.; Smith, R.; Shaw, D.; Mackay, J.; Alewood, P.; Christie, M.; King, Glenn. "Discovery and structure of a potent and highly specific blocker of insect calcium channels."  J. Biol. Chem. 276, 40306-40312 (2001).

Assembly members:
omega-atracotoxin-Hv2a, polymer, 45 residues, 4478 Da.

Natural source:   Common Name: Blue Mountains funnel-web spider   Taxonomy ID: 6904   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche versuta

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
omega-atracotoxin-Hv2a: LLACLFGNGRCSSNRDCCEL TPVCKRGSCVSSGPGLVGGI LGGIL

Data sets:
Data typeCount
1H chemical shifts280

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1omega-ACTX-Hv2a monomer1

Entities:

Entity 1, omega-ACTX-Hv2a monomer 45 residues - 4478 Da.

1   LEULEUALACYSLEUPHEGLYASNGLYARG
2   CYSSERSERASNARGASPCYSCYSGLULEU
3   THRPROVALCYSLYSARGGLYSERCYSVAL
4   SERSERGLYPROGLYLEUVALGLYGLYILE
5   LEUGLYGLYILELEU

Samples:

omega-Hv2a_sample_1: omega-atracotoxin-Hv2a 1.6 mM; chloramphenicol 25 microM; TSP 0.1 mM; D2O 5%

Ex-cond_1: ionic strength: 0.005 M; pH: 4.71; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H DQF-COSYnot availablenot availablenot available
2D 1H-1H E.COSYnot availablenot availablenot available

Software:

XEASY v1.3.13 - spectral analysis

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
SP P82852