BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4927

Title: 1H, 13C, 15N Resonance Assignments of a Circular Permuted Variant of CV-N (cpCV-N)

Authors: Barrientos, Laura; Campos-Olivas, Ramon; Gronenborn, Angela

Citation: Barrientos, Laura; Campos-Olivas, Ramon; Louis, John; Fiser, Andras; Sali, Andrej; Gronenborn, Angela. "Letter to the editor: 1H, 13C, 15N Resonance Assignments and Fold Verification of a Circular Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N"  J. Biomol. NMR 19, 289-290 (2001).

Assembly members:
Cyanovirin-N, polymer, 102 residues, 11013 Da.

Natural source:   Common Name: Cyanobacterium   Taxonomy ID: not available   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Nostoc ellipsosporum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Cyanovirin-N: GLGKFIETCRNTQLAGSSEL AAECKTRAQQFVSTKINLDD HIANIDGTLKWQPSNFSQTC YNSAIQGSVLTSTCERTNGG YNTSSIDLNSVIENVDGSLK YE

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts423
15N chemical shifts116
1H chemical shifts632

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Circular Permuted Cyanovirin-N, cpCV-N monomer1

Entities:

Entity 1, Circular Permuted Cyanovirin-N, cpCV-N monomer 102 residues - 11013 Da.

1   GLYLEUGLYLYSPHEILEGLUTHRCYSARG
2   ASNTHRGLNLEUALAGLYSERSERGLULEU
3   ALAALAGLUCYSLYSTHRARGALAGLNGLN
4   PHEVALSERTHRLYSILEASNLEUASPASP
5   HISILEALAASNILEASPGLYTHRLEULYS
6   TRPGLNPROSERASNPHESERGLNTHRCYS
7   TYRASNSERALAILEGLNGLYSERVALLEU
8   THRSERTHRCYSGLUARGTHRASNGLYGLY
9   TYRASNTHRSERSERILEASPLEUASNSER
10   VALILEGLUASNVALASPGLYSERLEULYS
11   TYRGLU

Samples:

sample_1: Cyanovirin-N, [U-15N], 0.3 – 1 mM; phosphate buffer 20 mM

sample_2: Cyanovirin-N, [U-15N; U-13C], 0.3 – 1.0 mM; phosphate buffer 20 mM

Overall_conditions: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQC/HMQCnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D DIPSI-H(CCO)NHnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D 15N-edited NOESYnot availablenot availablenot available
4D 13C,13C NOESYnot availablenot availablenot available

Software:

NMRView v4.1.1 -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

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