BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4929

Title: The 1H, 15N and 13C resonance assignments for the Tctex1 dynein light chain from Chlamydomonas flagella

Authors: Wu, Hongwei; Maciejewski, Mark; Benashski, Sharon; Mullen, Gregory; King, Stephen

Citation: Wu, Hongwei; Maciejewski, Mark; Benashski, Sharon; Mullen, Gregory; King, Stephen. "Letter to the Editor: 1H, 15N and 13C resonance assignments for the Tctex1 dynein light chain from Chlamydomonas flagella"  J. Biomol. NMR 20, 89-90 (2001).

Assembly members:
Chlamydomonas Tctex1, polymer, 114 residues, 12668 Da.

Natural source:   Common Name: Chlamydomonas reinhardtii   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Chlamydomonas Tctex1: MEGVDPAVEEAAFVADDVSN IIKESIDAVLQNQQYSEAKV SQWTSSCLEHCIKRLTALNK PFKYVVTCIIMQKNGAGLHT AASCWWDSTTDGSRTVRWEN KSMYCICTVFGLAI

Data sets:
Data typeCount
1H chemical shifts391
13C chemical shifts403
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tctex1 subunit 11
2Tctex1 subunit 21

Entities:

Entity 1, Tctex1 subunit 1 114 residues - 12668 Da.

1   METGLUGLYVALASPPROALAVALGLUGLU
2   ALAALAPHEVALALAASPASPVALSERASN
3   ILEILELYSGLUSERILEASPALAVALLEU
4   GLNASNGLNGLNTYRSERGLUALALYSVAL
5   SERGLNTRPTHRSERSERCYSLEUGLUHIS
6   CYSILELYSARGLEUTHRALALEUASNLYS
7   PROPHELYSTYRVALVALTHRCYSILEILE
8   METGLNLYSASNGLYALAGLYLEUHISTHR
9   ALAALASERCYSTRPTRPASPSERTHRTHR
10   ASPGLYSERARGTHRVALARGTRPGLUASN
11   LYSSERMETTYRCYSILECYSTHRVALPHE
12   GLYLEUALAILE

Samples:

Tctex1_dimer: Chlamydomonas Tctex1, [U-97% 13C; U-99% 15N], 1.4 mM

condition_1: pH: 6.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCA (6388 x 4400 x 1418 Hz, 512 x 55 x 30, 48 scans)not availablenot availablenot available
HN(CO)CA (6388 x 4400 x 141 8 Hz, 512 x 55 x 30, 48 scans)not availablenot availablenot available
HNCACB (6388 x 8000 x 1418 Hz, 512 x 50 x 32, 64 scans)not availablenot availablenot available
CBCA(CO)NH (6388 x 8000 x 1418 Hz, 512 x 46 x 31, 64 scans)not availablenot availablenot available
HNCO (6388 x 2200 x 1418 Hz, 512 x 48 x 29, 32 scans)not availablenot availablenot available
(HACA)CO(CA)NH (6388 x 2200 x 1418 Hz, 512 x 48 x 28, 80 scans)not availablenot availablenot available
HNHA (7654 x 7654 x 1700 Hz, 512 x 60 x 28, 48 scans)not availablenot availablenot available
3D 1H-15N NOESY-HSQC (7654 x 7654 x 1700 Hz, 512 x 96 x 32, 32 scans)not availablenot availablenot available
C(CO)NH-TOCSY (6388 x 9000 x 1418 Hz, 512 x 96 x 28, 48 scans)not availablenot availablenot available
HC(CO)NH-TOCSY (6388 x 6388 x 1418 Hz, 512 x 72 x 28, 64 scans)not availablenot availablenot available
HACA(CO)CANH (6388 x 2200 x 1418 Hz, 512 x 48 x 28, 80 scans).not availablenot availablenot available
2D 1H-15N HSQC (6388 x 1418 Hz, 512 x 80, 16 scans)not availablenot availablenot available
2D 1H-13C HSQC (7654 x 12000 Hz, 512 x 400, 64 scans)not availablenot availablenot available

Software:

FELIX v97.0 - processing spectra

XEASY v1.3.13 - assignments

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAC18035 EDO97227
REF XP_001702138