BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4935

Title: Backbone Assignments for Af19

Authors: Pakhomova, Olga; Cui, Yumin; Zwieb, Christian; Hinck, Andrew

Citation: Pakhomova, Olga; Cui, Yumin; Zwieb, Christian; Hinck, Andrew. "Letter to the Editor: Sequence-specific 1H, 13C, and 15N signal assignments and secondary structure of Archaeoglobus fulgidus SRP19"  J. Biomol. NMR 20, 187-188 (2001).

Assembly members:
Archaeglobus fulgidus SRP19, polymer, 104 residues, 12372.6 Da.

Natural source:   Common Name: Archaeoglobus fulgidus   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: not available   Genus/species: Archaeoglobus fulgidus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Archaeglobus fulgidus SRP19: MKESVVWTVNLDSKKSRAEG RRIPRRFAVPNVKLHELVEA SKELGLKFRAEEKKYPKSWW EEGGRVVVEKRGTKTKLMIE LARKIAEIREQKREQKKDKK KKKK

Data sets:
Data typeCount
1H chemical shifts687
13C chemical shifts425
15N chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Af19 monomer1

Entities:

Entity 1, Af19 monomer 104 residues - 12372.6 Da.

1   METLYSGLUSERVALVALTRPTHRVALASN
2   LEUASPSERLYSLYSSERARGALAGLUGLY
3   ARGARGILEPROARGARGPHEALAVALPRO
4   ASNVALLYSLEUHISGLULEUVALGLUALA
5   SERLYSGLULEUGLYLEULYSPHEARGALA
6   GLUGLULYSLYSTYRPROLYSSERTRPTRP
7   GLUGLUGLYGLYARGVALVALVALGLULYS
8   ARGGLYTHRLYSTHRLYSLEUMETILEGLU
9   LEUALAARGLYSILEALAGLUILEARGGLU
10   GLNLYSARGGLUGLNLYSLYSASPLYSLYS
11   LYSLYSLYSLYS

Samples:

sample1: Archaeglobus fulgidus SRP19, [U-99% 13C; U-98% 15N], 1.8 – 2.2 mM; NaCl 0.050 M; KH2PO4 0.025 M; D2O 99.88%

sample2: Archaeglobus fulgidus SRP19, [U-98% 15N], 1.8 – 2.2 mM; NaCl 0.050 M; KH2PO4 0.025 M; D2O 5%; H2O 95%

condition1: pH: 6.0; temperature: 300 K; ionic strength: 0.075 M

Experiments:

NameSampleSample stateSample conditions
2D 1H/15N HSQCnot availablenot availablecondition1
2D 1H/13C CT-HSQCnot availablenot availablecondition1
3D CBCA(CO)NHnot availablenot availablecondition1
3D HNCACBnot availablenot availablecondition1
3D CCONHnot availablenot availablecondition1
3D HBHA(CO)NHnot availablenot availablecondition1
3D HNCOnot availablenot availablecondition1
3D HN(CA)COnot availablenot availablecondition1
3D HCCH-TOCYnot availablenot availablecondition1

Software:

nmrPipe v1.7 - processing, apodization, zero filling, fourier transformation

PIPP v4.3.1 - Analysis, peak picking

XEASY v1.3.12 - analysis, peak picking, sequential assignment

NMR spectrometers:

  • Bruker AMX2 500 MHz

Related Database Links:

PDB
GB AAB89988 AIG98129
REF NP_070086 WP_010878753
SP O29010