BMRB Entry 4945
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4945
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Title: VAM3P N-TERMINAL DOMAIN SOLUTION STRUCTURE PubMed: 11224573
Authors: Dulubova, I.; Yamaguchi, T.; Wang, Y.; Sudhof, T.; Rizo, J.
Citation: Dulubova, I.; Yamaguchi, T.; Wang, Y.; Sudhof, T.; Rizo, J.. "Vam3p Structure Reveals Conserved and Divergent Properties of Syntaxins" Nat. Struct. Biol. 8, 258-264 (2001).
Assembly members:
single chain biopolimer, polymer, 123 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
single chain biopolimer: GSSQQEPQFSTNQKTKELSN
LIETFAEQSRVLEKECTKIG
SKRDSKELRYKIETELIPNC
TSVRDKIESNILIHQNGKLS
ADFKNLKTKYQSLQQSYNQR
KSLFPLKTPISPGTSKERKD
IHP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 869 |
| 13C chemical shifts | 502 |
| 15N chemical shifts | 126 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Vam3p N-terminal domain | 1 |
Entities:
Entity 1, Vam3p N-terminal domain 123 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLN | GLN | GLU | PRO | GLN | PHE | SER | ||||
| 2 | THR | ASN | GLN | LYS | THR | LYS | GLU | LEU | SER | ASN | ||||
| 3 | LEU | ILE | GLU | THR | PHE | ALA | GLU | GLN | SER | ARG | ||||
| 4 | VAL | LEU | GLU | LYS | GLU | CYS | THR | LYS | ILE | GLY | ||||
| 5 | SER | LYS | ARG | ASP | SER | LYS | GLU | LEU | ARG | TYR | ||||
| 6 | LYS | ILE | GLU | THR | GLU | LEU | ILE | PRO | ASN | CYS | ||||
| 7 | THR | SER | VAL | ARG | ASP | LYS | ILE | GLU | SER | ASN | ||||
| 8 | ILE | LEU | ILE | HIS | GLN | ASN | GLY | LYS | LEU | SER | ||||
| 9 | ALA | ASP | PHE | LYS | ASN | LEU | LYS | THR | LYS | TYR | ||||
| 10 | GLN | SER | LEU | GLN | GLN | SER | TYR | ASN | GLN | ARG | ||||
| 11 | LYS | SER | LEU | PHE | PRO | LEU | LYS | THR | PRO | ILE | ||||
| 12 | SER | PRO | GLY | THR | SER | LYS | GLU | ARG | LYS | ASP | ||||
| 13 | ILE | HIS | PRO |
Samples:
sample_1: single chain biopolimer, [U-99% 15N], 0.9 mM; phosphate buffer 20 mM
sample_2: single chain biopolimer, [U-99% 13C; U-99% 15N], 0.85 mM; phosphate buffer 20 mM
sample_cond_1: pH: 6.0; temperature: 303 K; ionic strength: 20 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
Software:
VNMR v6.1B - collection
NMRPipe v1.7 - processing
NMRView v4.1 - data analysis
CNS v0.9 - structure solution
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz