BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4950

Title: Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein

Authors: Geyer, Matthias; Kalbitzer, Hans

Citation: Geyer, Matthias; Munte, Claudia; Schorr, Jacqueline; Kellner, Roland; Kalbitzer, Hans. "Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein"  J. Mol. Biol. 289, 123-138 (1999).

Assembly members:
HIV-1 Negative Factor, polymer, 57 residues, Formula weight is not available

Natural source:   Common Name: Human immunodeficiency virus type 1   Taxonomy ID: 11676   Superkingdom: viruses   Kingdom: not available   Genus/species: HIV-1 BEN allele (Nef_Hv112)

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HIV-1 Negative Factor: GGKWSKSSVVGWPAVRERMR RAEPAADGVGAASRDLEKHG AITSSNTAANNAACAWX

Data sets:
Data typeCount
1H chemical shifts322

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1non-myristoylated Nef anchor domain (2-57)1

Entities:

Entity 1, non-myristoylated Nef anchor domain (2-57) 57 residues - Formula weight is not available

1   GLYGLYLYSTRPSERLYSSERSERVALVAL
2   GLYTRPPROALAVALARGGLUARGMETARG
3   ARGALAGLUPROALAALAASPGLYVALGLY
4   ALAALASERARGASPLEUGLULYSHISGLY
5   ALAILETHRSERSERASNTHRALAALAASN
6   ASNALAALACYSALATRPNH2

Samples:

sample_1: HIV-1 Negative Factor 1.0 mM

Ex-cond_1: pH: 4.6; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYsample_1not availableEx-cond_1
TOCSYsample_1not availableEx-cond_1
COSYsample_1not availableEx-cond_1

Software:

AURELIA - automated peak assignments

NMR spectrometers:

  • Bruker DMX Avance 800 MHz
  • Bruker AMX 500 MHz

Related Database Links:

BMRB 4951
PDB
DBJ BAC02670 BAC02671
EMBL CAA26947
GB AAA44206 AAA44680 AAA45001 AAB59874 AAD02461
PIR S03245
PRF 1103299A
SP P03404 P04324 P05854