BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4951

Title: Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein

Authors: Geyer, Matthias; Munte, Claudia; Kalbitzer, Hans

Citation: Geyer, Matthias; Munte, Claudia; Schorr, Jacqueline; Kellner, Roland; Kalbitzer, Hans. "Structure of the Anchor-Domain of Myristoylated and Non-myristoylated HIV-1 Nef Protein"  J. Mol. Biol. 289, 123-138 (1999).

Assembly members:
HIV-1 Negative Factor, polymer, 58 residues, 6022 Da.

Natural source:   Common Name: Human immunodeficiency virus type 1   Taxonomy ID: 11676   Superkingdom: viruses   Kingdom: not available   Genus/species: HIV-1 BEN allele (Nef_Hv112)

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HIV-1 Negative Factor: XGGKWSKSSVVGWPAVRERM RRAEPAADGVGAASRDLEKH GAITSSNTAANNAACAWX

Data sets:
Data typeCount
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myristoylated Nef anchor domain (Myr-2-57)1

Entities:

Entity 1, myristoylated Nef anchor domain (Myr-2-57) 58 residues - 6022 Da.

1   MYRGLYGLYLYSTRPSERLYSSERSERVAL
2   VALGLYTRPPROALAVALARGGLUARGMET
3   ARGARGALAGLUPROALAALAASPGLYVAL
4   GLYALAALASERARGASPLEUGLULYSHIS
5   GLYALAILETHRSERSERASNTHRALAALA
6   ASNASNALAALACYSALATRPNH2

Samples:

sample_1: HIV-1 Negative Factor 0.37 mM

Ex-cond_1: pH: 4.6; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYsample_1not availableEx-cond_1
ROESYsample_1not availableEx-cond_1
TOCSYsample_1not availableEx-cond_1
COSYsample_1not availableEx-cond_1

Software:

AURELIA - automated peak assignments

NMR spectrometers:

  • Bruker DMX Avance 800 MHz
  • Bruker AMX 500 MHz

Related Database Links:

BMRB 4950
PDB
DBJ BAC02670.1 BAC02671.1
EMBL CAA26947.1
GenBank AAA44206.1 AAA45001.1 AAB59874.1 AAD02461.1
PIR S03245
PRF 1103299A
SWISS-PROT P04324 P05854 P03404