BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4954

Title: 1H, 13C and 15N chemical shifts assignments for the N-terminal domain of riboflavin synthase of E. Coli with riboflavin as a bound ligand

Authors: Truffault, Vincent; Coles, Murray; Diercks, Tammo; Abelmann, Kerstin; Eberhardt, Sabine; Luettgen, Holger; Bacher, Adelbert; Kessler, Horst

Citation: Truffault, Vincent; Coles, Murray; Diercks, Tammo; Abelmann, Kerstin; Eberhardt, Sabine; Luettgen, Holger; Bacher, Adelbert; Kessler, Horst. "The Solution Strucutre of the N-terminal Domain of Riboflavin Synthase"  J. Mol. Biol. 309, 949-960 (2001).

Assembly members:
Riboflavin Synthase, polymer, 97 residues, Formula weight is not available
RIBOFLAVIN, non-polymer, 376.364 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Riboflavin Synthase: MFTGIVQGTAKLVSIDEKPN FRTHVVELPDHMLDGLETGA SVAHNGCCLTVTEINGNHVS FDLMKETLRITNLGDLKVGD WVNVERAAKFSDEIGGH

Data sets:
Data typeCount
1H chemical shifts661
13C chemical shifts397
15N chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Riboflavin Synthase subunit 11
2Riboflavin Synthase subunit 21
3Riboflavin 12
4Riboflavin 22

Entities:

Entity 1, Riboflavin Synthase subunit 1 97 residues - Formula weight is not available

1   METPHETHRGLYILEVALGLNGLYTHRALA
2   LYSLEUVALSERILEASPGLULYSPROASN
3   PHEARGTHRHISVALVALGLULEUPROASP
4   HISMETLEUASPGLYLEUGLUTHRGLYALA
5   SERVALALAHISASNGLYCYSCYSLEUTHR
6   VALTHRGLUILEASNGLYASNHISVALSER
7   PHEASPLEUMETLYSGLUTHRLEUARGILE
8   THRASNLEUGLYASPLEULYSVALGLYASP
9   TRPVALASNVALGLUARGALAALALYSPHE
10   SERASPGLUILEGLYGLYHIS

Entity 2, Riboflavin 1 - C17 H20 N4 O6 - 376.364 Da.

1   RBF

Samples:

protein_labelled: Riboflavin Synthase, [U-13C; U-15N], 1.0 mM; RIBOFLAVINE mM

riboflavin_labelled: Riboflavin Synthase, [U-15N], 1.0 mM; RIBOFLAVINE, [U-13C; U-15N], mM

general: pH: 7.3; temperature: 300 K; ionic strength: 0.125 M

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HCACOnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCH-COSYnot availablenot availablenot available
1H-15N-NOESYnot availablenot availablenot available
1H-13C-NOESYnot availablenot availablenot available
CNH-NOESYnot availablenot availablenot available
NNH-NOESYnot availablenot availablenot available
NCH-NOESYnot availablenot availablenot available
CCH-NOESYnot availablenot availablenot available
2D-13C-filtered,12C-edited-NOESYnot availablenot availablenot available
2D-12C-edited,13C-filtered-NOESYnot availablenot availablenot available

Software:

PASTA - automatic sequential assignment

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAA15429 BAB35794 BAG77310 BAI25639 BAI30613
EMBL CAA48861 CAP76161 CAQ32140 CAQ98571 CAR03023
GB AAB47940 AAC74734 AAG56651 AAN43269 AAN80516
REF NP_288098 NP_310398 NP_416179 NP_707562 NP_753951
SP P0AFU8 P0AFU9