BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4959

Title: Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin

Authors: Koshiba, Seizo; Kigawa, Takanori; Kikuchi, Akira; Yokoyama, Shigeyuki

Citation: Koshiba, Seizo; Kigawa, Takanori; Kikuchi, Akira; Yokoyama, Shigeyuki. "Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin"  J. Struct. Func. Genomics 2, 1-8 (2001).

Assembly members:
epsin ENTH domain, polymer, 148 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
epsin ENTH domain: GSSRMSTSSLRRQMKNIVHN YSEAEIKVREATSNDPWGPS SSLMSEIADLTYNVVAFSEI MSMIWKRLNDHGKNWRHVYK AMTLMEYLIKTGSERVSQQC KENMYAVQTLKDFQYVDRDG KDQGVNVREKAKQLVALLRD EDRLREER

Data sets:
Data typeCount
1H chemical shifts920
13C chemical shifts606
15N chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1epsin ENTH domain1

Entities:

Entity 1, epsin ENTH domain 148 residues - Formula weight is not available

1   GLYSERSERARGMETSERTHRSERSERLEU
2   ARGARGGLNMETLYSASNILEVALHISASN
3   TYRSERGLUALAGLUILELYSVALARGGLU
4   ALATHRSERASNASPPROTRPGLYPROSER
5   SERSERLEUMETSERGLUILEALAASPLEU
6   THRTYRASNVALVALALAPHESERGLUILE
7   METSERMETILETRPLYSARGLEUASNASP
8   HISGLYLYSASNTRPARGHISVALTYRLYS
9   ALAMETTHRLEUMETGLUTYRLEUILELYS
10   THRGLYSERGLUARGVALSERGLNGLNCYS
11   LYSGLUASNMETTYRALAVALGLNTHRLEU
12   LYSASPPHEGLNTYRVALASPARGASPGLY
13   LYSASPGLNGLYVALASNVALARGGLULYS
14   ALALYSGLNLEUVALALALEULEUARGASP
15   GLUASPARGLEUARGGLUGLUARG

Samples:

sample1: epsin ENTH domain, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 400 mM; dithiothreitol, [U-2H], 2 mM; sodium azide 0.01%; H2O 90%; D2O 10%

sample2: epsin ENTH domain, [U-15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 400 mM; dithiothreitol, [U-2H], 2 mM; sodium azide 0.01%; H2O 90%; D2O 10%

cond1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 15N HSQCnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
2D 13C HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available
3D H(CCO)NHnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D 13C NOESY-HSQCnot availablenot availablenot available
4D 13C/15N NOESYnot availablenot availablenot available
4D 13C/13C NOESYnot availablenot availablenot available

Software:

NMRPipe - data processing

NMRView - peak assignments

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAB14041 BAG62253 BAI45694
GB AAC33823 AAD38326 AAH44651 AAH46962 AAH67206
REF NP_001033759 NP_001123543 NP_001123544 NP_001239383 NP_034277
SP O88339 Q80VP1 Q9Y6I3
TPG DAA19370