BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4960

Title: Solution Conformation of the Met 61 to His 61 Mutant of Pseudomonas stutzeri substarin ZoBell Ferrocytochrome c-551

Authors: Miller, Gregory; Hardman, John; Timkovich, Russell

Citation: Miller, Gregory; Hardman, John; Timkovich, Russell. "Solution Conformation of the Met 61 to His 61 Mutant of Pseudomonas stutzeri ZoBell Foerrocytochrome c-551"  Biophys. J. 80, .-. (2001).

Assembly members:
cytochrome c-551, polymer, 82 residues, 9500 Da.
HEC, non-polymer, 618.503 Da.

Natural source:   Common Name: Pseudomonas stutzeri ZoBell   Taxonomy ID: 96564   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas stutzeri

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cytochrome c-551: QDGEALFKSKPCAACHSVDT KMVGPALKEVAAKNAGVEGA ADTLALHIKNGSQGVWGPIP HPPNPVTEEEAKILAEWVLS LK

Data sets:
Data typeCount
1H chemical shifts509

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M61H cyt c-5511
2heme c2

Entities:

Entity 1, M61H cyt c-551 82 residues - 9500 Da.

1   GLNASPGLYGLUALALEUPHELYSSERLYS
2   PROCYSALAALACYSHISSERVALASPTHR
3   LYSMETVALGLYPROALALEULYSGLUVAL
4   ALAALALYSASNALAGLYVALGLUGLYALA
5   ALAASPTHRLEUALALEUHISILELYSASN
6   GLYSERGLNGLYVALTRPGLYPROILEPRO
7   HISPROPROASNPROVALTHRGLUGLUGLU
8   ALALYSILELEUALAGLUTRPVALLEUSER
9   LEULYS

Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

literature_sample: cytochrome c-5514 – 5 mM; H2O 90%; D2O 10%; sodium borate buffer mM

literature_sample_conditions: pH: 9.2; temperature: 323 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
NOESYliterature_samplenot availableliterature_sample_conditions
DQF-COSYliterature_samplenot availableliterature_sample_conditions
HOHAHAliterature_samplenot availableliterature_sample_conditions

Software:

FELIX v95 -

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 7296
PDB
EMBL CAA40153
GB EHY75989
REF WP_003279940
SP P00101