BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4966

Title: 1H and 13C Chemical Shift Assignments for Cardiotoxin A3 from Naja Atra at Neutral pH   PubMed: 11669614

Authors: Sue, Shih-Che; Jarrell, Harold; Brisson, Jean-Robert; Wu, Wen-guey

Citation: Sue, Shih-Che; Jarrell, Harold; Brisson, Jean-Robert; Wu, Wen-guey. "Dynamic Characterization of the Water binding loop in the P-type Cardiotoxin: Implication for the role of the bound Water Molecule"  Biochemistry 40, 12782-12794 (2001).

Assembly members:
Cardiotoxin A3, polymer, 60 residues, 6741 Da.

Natural source:   Common Name: Taiwan cobra   Taxonomy ID: 8656   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: cobra naja atra

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Cardiotoxin A3: LKCNKLVPLFYKTCPAGKNL CYKMFMVATPKVPVKRGCID VCPKSSLLVKYVCCNTDRCN

Data sets:
Data typeCount
1H chemical shifts432
13C chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cardiotoxin A31

Entities:

Entity 1, cardiotoxin A3 60 residues - 6741 Da.

1   LEULYSCYSASNLYSLEUVALPROLEUPHE
2   TYRLYSTHRCYSPROALAGLYLYSASNLEU
3   CYSTYRLYSMETPHEMETVALALATHRPRO
4   LYSVALPROVALLYSARGGLYCYSILEASP
5   VALCYSPROLYSSERSERLEULEUVALLYS
6   TYRVALCYSCYSASNTHRASPARGCYSASN

Samples:

sample_1: Cardiotoxin A3 5 mM; phosphate buffer 10 mM

CTX_A3_condition: pH: 5.96; temperature: 300 K; ionic strength: 0.03 M; pressure: 1.013 atm

Experiments:

NameSampleSample stateSample conditions
COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

XWINNMR v2.01 -

NMR spectrometers:

  • BRUKER DRX 600 MHz
  • BRUKER DMX 500 MHz

Related Database Links:

BMRB 15305
PDB
EMBL CAA07686 CAA90963 CAB42053 CAB42055
GB AAA49386 AAB01541 AAB18382 AAB18383 AAB25733
PIR JK0222
PRF 0406231A 2207174B
SP O93471 O93473 P60301 P60302 P60303