BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4972

Title: 1H, 13C, and 15N assignment of Crh, a protein involved in carbon catabolite repression in B. subtilis   PubMed: 11916384

Authors: Favier, Adrien; Brutscher, Bernhard; Blackledge, M.; Galinier, Anne; Deutscher, J.; Penin, Francois; Marion, Dominique

Citation: Favier, Adrien; Brutscher, Bernhard; Blackledge, M.; Galinier, Anne; Deutscher, J.; Penin, Francois; Marion, Dominique. "Solution structure and dynamics of Crh, the Bacillus subtilis catabolite repression HPr"  J. Mol. Biol. 317, 131-144 (2002).

Assembly members:
catabolite repression HPr, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
catabolite repression HPr: MVQQKVEVRLKTGLQARPAA LFVQEANRFTSDVFLEKDGK KVNAKSIMGLMSLAVSTGTE VTLIAQGEDEQEALEKLAAY VQEEVLQ

Data sets:
Data typeCount
1H chemical shifts604
13C chemical shifts354
15N chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Crh1

Entities:

Entity 1, Crh 87 residues - Formula weight is not available

1   METVALGLNGLNLYSVALGLUVALARGLEU
2   LYSTHRGLYLEUGLNALAARGPROALAALA
3   LEUPHEVALGLNGLUALAASNARGPHETHR
4   SERASPVALPHELEUGLULYSASPGLYLYS
5   LYSVALASNALALYSSERILEMETGLYLEU
6   METSERLEUALAVALSERTHRGLYTHRGLU
7   VALTHRLEUILEALAGLNGLYGLUASPGLU
8   GLNGLUALALEUGLULYSLEUALAALATYR
9   VALGLNGLUGLUVALLEUGLN

Samples:

sample_1: catabolite repression HPr, [U-95% 13C; U-90% 15N], 0.4 – 0.5 mM

sample_2: catabolite repression HPr, [U-90% 15N], 0.4 – 0.5 mM

Ex-cond_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-edited NOESY-HSQCnot availablenot availablenot available
3D 15N-edited TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
H(C)C(CO)NH-TOCSYnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available
H(C)CH-TOCSYnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • VARIAN INOVA 600 MHz
  • VARIAN INOVA 800 MHz

Related Database Links:

BMRB 5757
PDB
DBJ BAI87096 BAM55550 BAM59563 GAK79281
EMBL CAB08060 CAB15479 CCU60557 CEI59278 CEJ79135
GB ADM39440 ADV94285 AEP88381 AEP92509 AFI30049
REF NP_391354 WP_003219835 WP_024122965 WP_024713407 YP_003867749
SP O06976