BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4977

Title: 1H and 15N NMR assignments of the 7kd wheat lipid transfer protein

Authors: Pons, Jean-Luc; Delsuc, Marc-Andre

Citation: Pons, Jean-Luc; Delsuc, Marc-Andre. "1H and 15N NMR assignments of the 7kd wheat lipid transfer protein"  The BMRB entry is the only known published source for the data..

Assembly members:
lipid transfer protein, polymer, 67 residues, 6980 Da.

Natural source:   Common Name: wheat   Taxonomy ID: 4565   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Triticum vulgare

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
lipid transfer protein: ACQASQLAVCASAILSGAKP SGECCGNLRAQQGCFCQYAK DPTYGQYIRSPHARDTLTSC GLAVPHC

Data sets:
Data typeCount
15N chemical shifts61
1H chemical shifts421

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LTP1

Entities:

Entity 1, LTP 67 residues - 6980 Da.

1   ALACYSGLNALASERGLNLEUALAVALCYS
2   ALASERALAILELEUSERGLYALALYSPRO
3   SERGLYGLUCYSCYSGLYASNLEUARGALA
4   GLNGLNGLYCYSPHECYSGLNTYRALALYS
5   ASPPROTHRTYRGLYGLNTYRILEARGSER
6   PROHISALAARGASPTHRLEUTHRSERCYS
7   GLYLEUALAVALPROHISCYS

Samples:

sample_1: lipid transfer protein, [U-15N], 2.8 mM

Ex-cond_1: pH: 3.5; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H COSY (3 TMP)sample_1not availableEx-cond_1
1H TOCSY (3 TMP)sample_1not availableEx-cond_1
1H NOESY (3 TMP)sample_1not availableEx-cond_1
1H-15N HSQC-TOCSYsample_1not availableEx-cond_1
1H-15N HSQC-NOESYsample_1not availableEx-cond_1

Software:

Gifa assignment module v1.3 - Assignment

RESCUE v1.0 - Assignment Aid Tool

NMR spectrometers:

  • BRUKER AMX 600 MHz

Related Database Links:

SWISS-PROT P82900
EMBL CAH69203 CAH69202 CAH69200 CAC13149
PDB