BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4979

Title: 1H and 13C chemical shift assignments for the pheromone Er-23 from Euplotes raikovi   PubMed: 11700049

Authors: Zahn, Ralph; Damberger, Fred; Ortenzi, Claudio; Luporini, Pierangelo; Wuthrich, Kurt

Citation: Zahn, Ralph; Damberger, Fred; Ortenzi, Claudio; Luporini, Pierangelo; Wuthrich, Kurt. "Communication: NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds"  J. Mol. Biol. 313, 923-931 (2001).

Assembly members:
Er-23 pheromone, polymer, 51 residues, 5095 Da.

Natural source:   Common Name: Euplotes raikovi   Taxonomy ID: 5938   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Euplotes raikovi

Experimental source:   Production method: purified from the natural source   Host organism: Euplotes raikovi

Entity Sequences (FASTA):
Er-23 pheromone: GECEQCFSDGGDCTTCFNNG TGPCANCLAGYPAGCSNSDC TAFLSQCYGGC

Data sets:
Data typeCount
13C chemical shifts51
1H chemical shifts267

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Er-231

Entities:

Entity 1, Er-23 51 residues - 5095 Da.

1   GLYGLUCYSGLUGLNCYSPHESERASPGLY
2   GLYASPCYSTHRTHRCYSPHEASNASNGLY
3   THRGLYPROCYSALAASNCYSLEUALAGLY
4   TYRPROALAGLYCYSSERASNSERASPCYS
5   THRALAPHELEUSERGLNCYSTYRGLYGLY
6   CYS

Samples:

sample_1: Er-23 pheromone 1.0 mM; H2O 90%; D2O 10%

sample_2: Er-23 pheromone 1.0 mM; D2O 100%

cond_1: pH: 4.6; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
E.COSY, 2Q-COSY & 1H-13C HSQC recorded in D2Onot availablenot availablecond_1
NOESY in H2Onot availablenot availablecond_1
ZQ-suppressed NOESY in D2Onot availablenot availablecond_1
other experiments recorded in H2O & D2Onot availablenot availablecond_1

Software:

XEASY - assisted resonance assignment

DYANA - automatic NOESY chemical shift assignment, automatic structure calculation

OPAL - Structure Refinement in simulated H2O bath

NMR spectrometers:

  • Varian Unity-plus 750 MHz
  • Bruker AMX 600 MHz

Related Database Links:

PDB
SP P58547