BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4980

Title: 1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease

Authors: Crescenzi, Orlando; Carotenuto, Alfonso; D'Ursi, Anna; Tancredi, Teodorico; D'Alessio, Giuseppe; Avitabile, Francesca; Picone, Delia

Citation: Crescenzi, Orlando; Carotenuto, Alfonso; D'Ursi, Anna; Tancredi, Teodorico; D'Alessio, Giuseppe; Avitabile, Francesca; Picone, Delia. "Letter to the Editor: 1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease"  J. Biomol. NMR 20, 289-290 (2001).

Assembly members:
bovine seminal ribonuclease monomer, polymer, 125 residues, Formula weight is not available
GLUTATHIONE, non-polymer, 307.323 Da.

Natural source:   Common Name: bull   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
bovine seminal ribonuclease monomer: MKESAAAKFERQHMDSGNSP SSSSNYCNLMMCCRKMTQGK CKPVNTFVHESLADVKAVCS QKKVTCKDGQTNCYQSKSTM RITDCRETGSSKYPNCAYKT TQVEKHIIVACGGKPSVPVH FDASV

Data sets:
Data typeCount
15N chemical shifts129
1H chemical shifts710

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ribonuclease1
2glutathione_12
3glutathione_22

Entities:

Entity 1, ribonuclease 125 residues - Formula weight is not available

1   METLYSGLUSERALAALAALALYSPHEGLU
2   ARGGLNHISMETASPSERGLYASNSERPRO
3   SERSERSERSERASNTYRCYSASNLEUMET
4   METCYSCYSARGLYSMETTHRGLNGLYLYS
5   CYSLYSPROVALASNTHRPHEVALHISGLU
6   SERLEUALAASPVALLYSALAVALCYSSER
7   GLNLYSLYSVALTHRCYSLYSASPGLYGLN
8   THRASNCYSTYRGLNSERLYSSERTHRMET
9   ARGILETHRASPCYSARGGLUTHRGLYSER
10   SERLYSTYRPROASNCYSALATYRLYSTHR
11   THRGLNVALGLULYSHISILEILEVALALA
12   CYSGLYGLYLYSPROSERVALPROVALHIS
13   PHEASPALASERVAL

Entity 2, glutathione_1 - C10 H17 N3 O6 S - 307.323 Da.

1   GSH

Samples:

15N-labelled: bovine seminal ribonuclease monomer, [U-97% 15N], 2.0 mM; GLUTATHIONE 4.0 mM

standard: pH: 5.65; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSY15N-labellednot availablestandard
2D 1H-1H DQ-COSY15N-labellednot availablestandard
2D 1H-1H NOESY15N-labellednot availablestandard
2D 1H-1H TOCSY15N-labellednot availablestandard
2D 1H-1H CLEAN-TOCSY15N-labellednot availablestandard
2D 1H-15N HSQC15N-labellednot availablestandard
3D 1H-1H-15N NOESY15N-labellednot availablestandard
3D 1H-1H-15N TOCSY15N-labellednot availablestandard
3D HNHA15N-labellednot availablestandard
3D HNHB15N-labellednot availablestandard

Software:

nmrPipe - data processing

nmrView v4.0.3 - spectra visualization

dyana v1.5 - simulated annealing

amber v5.0 - restrained minimization

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA04155 CAA35716
GenBank AAB27820 AAB36140
REF NP_861526
SWISS-PROT P00669