BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4988

Title: Three Dimensional Solution Structure of Huwentoxin-II BY 2D 1H-NMR

Authors: Shu, Qin; Lu, Shan-yun; Gu, Xiao-chen; Liang, Song-ping

Citation: Shu, Q.; Lu, S.; Gu, X.; Liang, S.. "Three-dimensional Solution Structure Determination of Huwentoxin-II by 2D 1H-NMR"  . ., .-..

Assembly members:
Huwentoxin-II, polymer, 37 residues, 4284.3 Da.

Natural source:   Common Name: Chinese bird spider   Taxonomy ID: 29017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Selenocosmia huwena

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Huwentoxin-II: LFECSFSCEIEKEGDKPCKK KKCKGGWKCKFNMCVKV

Data sets:
Data typeCount
1H chemical shifts267

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HUWENTOXIN-II(Ile, 10)1

Entities:

Entity 1, HUWENTOXIN-II(Ile, 10) 37 residues - 4284.3 Da.

1   LEUPHEGLUCYSSERPHESERCYSGLUILE
2   GLULYSGLUGLYASPLYSPROCYSLYSLYS
3   LYSLYSCYSLYSGLYGLYTRPLYSCYSLYS
4   PHEASNMETCYSVALLYSVAL

Samples:

sample_1: Huwentoxin-II 4 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_2: Huwentoxin-II 4 mM; phosphate buffer 20 mM; D2O 100%

sample_cond_1: pH: 5.4; temperature: 300 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

FELIX v98.0 - data analysis

X-PLOR v3.851 - refinement

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
GB AAP33076 ABY77725 ABY77726 ABY77727 ABY77728
SP P68421 P82959 P82960