BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4991

Title: Identification of a novel archaebacterial thioredoxin: Determination of function through structure.   PubMed: 11939770

Authors: Bhattacharyya, Sudeepa; Habibi-Nazhad, Bahram; Amegbey, Godwin; Slupsky, Carolyn; Yee, Adelinda; Arrowsmith, Cheryl; Wishart, David

Citation: Bhattacharyya, Sudeepa; Habibi-Nazhad, Bahram; Amegbey, Godwin; Slupsky, Carolyn; Yee, Adelinda; Arrowsmith, Cheryl; Wishart, David. "Identification of a Novel Archaebacterial Thioredoxin: Determination of Function through Structure"  Biochemistry 41, 4760-4770 (2002).

Assembly members:
thioredoxin from Methanobacterium thermoautotrophicum delta H, polymer, 77 residues, 8500 Da.

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanothermobacter thermautotrophicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
thioredoxin from Methanobacterium thermoautotrophicum delta H: MMKIQIYGTGCANCQMLEKN AREAVKELGIDAEFEKIKEM DQILEAGLTALPGLAVDGEL KIMGRVASKEEIKKILS

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts78
1H chemical shifts442
coupling constants57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MtH8951

Entities:

Entity 1, MtH895 77 residues - 8500 Da.

1   METMETLYSILEGLNILETYRGLYTHRGLY
2   CYSALAASNCYSGLNMETLEUGLULYSASN
3   ALAARGGLUALAVALLYSGLULEUGLYILE
4   ASPALAGLUPHEGLULYSILELYSGLUMET
5   ASPGLNILELEUGLUALAGLYLEUTHRALA
6   LEUPROGLYLEUALAVALASPGLYGLULEU
7   LYSILEMETGLYARGVALALASERLYSGLU
8   GLUILELYSLYSILELEUSER

Samples:

sample_1: thioredoxin from Methanobacterium thermoautotrophicum delta H, [U-13C; U-15N], 1.0 mM

NMR-condition: ionic strength: 0.20 M; pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAM70061
GB AAB85393
REF NP_276032 WP_010876528
SP O26981