BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4995

Title: Solution NMR Structure and Folding Dynamics of the N-terminus of a rat Non-muscle Alpha-tropomyosin in an Engineered Chimeric Protein   PubMed: 11575936

Authors: Greenfield, Norma; Huang, Yuanpeng; Palm, Thomas; Swapna, G.V.T.S.; Monleon, Daniel; Montelione, Gaetano; Hitchcock-DeGregori, Sarah

Citation: Greenfield, Norma; Huang, Yuanpeng; Palm, Thomas; Swapna, G.V.T.S.; Monleon, Daniel; Montelione, Gaetano; Hitchcock-DeGregori, Sarah. "Solution NMR Structure and Folding Dynamics of the N-terminus of a rat Non-muscle Alpha-tropomyosin in an Engineered Chimeric Protein"  J. Mol. Biol. 312, 833-847 (2001).

Assembly members:
GlyTM1bZip, polymer, 38 residues, 8644 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
GlyTM1bZip: GAGSSSLEAVRRKIRSLQEQ NYHLENEVARLKKLVGER

Data sets:
Data typeCount
1H chemical shifts274
13C chemical shifts160
15N chemical shifts41
coupling constants34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlyTM1bZip subunit A1
2GlyTM1bZip subunit B1

Entities:

Entity 1, GlyTM1bZip subunit A 38 residues - 8644 Da.

1   GLYALAGLYSERSERSERLEUGLUALAVAL
2   ARGARGLYSILEARGSERLEUGLNGLUGLN
3   ASNTYRHISLEUGLUASNGLUVALALAARG
4   LEULYSLYSLEUVALGLYGLUARG

Samples:

GlyTM1bZip_(two-chained): GlyTM1bZip coiled-coil dimer 0.6 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

15N-GlyTM1bZip: GlyTM1bZip coiled-coil dimer, [U-99% 15N], 0.5 – 2.0 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

15N_13C-GlyTM1bZip: GlyTM1bZip coiled-coil dimer, [U-99% 13C; U-99% 15N], 0.8 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

GlyTM1bZip_heterodimer: GlyTM1bZip coiled-coil dimer 1.32 mM; GlyTM1bZip coiled-coil dimer, [U-99% 13C; U-99% 15N], 0.88 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.4; temperature: 281 K; ionic strength: 0.12 M

Experiments:

NameSampleSample stateSample conditions
15N-1H HSQCnot availablenot availablenot available
13C-!H HSQCnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
2QF-COSYnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CANHnot availablenot availablenot available
H(CA)NHnot availablenot availablenot available
H(CA)(CO)NHnot availablenot availablenot available
(CA)(C0)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CNCA(CO)NHnot availablenot availablenot available
H_HCC(CO)NH-TOCSYnot availablenot availablenot available
C_HCC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
15N edited PFG NOESYnot availablenot availablenot available
13C edited PFG NOESYnot availablenot availablenot available
13C X-filtered NOESYnot availablenot availablenot available
13C-15N X-filtered NOESYnot availablenot availablenot available

Software:

VNMR v5.3B - Data Processing

SPARKY v3.74 - Peak Picking

AutoAssign v1.5 - Automatic Assignments

AutoStructure v1.0 - Automatic Assignments of NOESY peaks, Structure determination

Dyana v1.5 - Structure determination using torsion angle dynamics

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 15965
PDB
DBJ BAD92278 BAG11063 BAH12832 BAH13458
GB AAA18098 AAA18099 AAA42253 AAH50473 AAH53545
REF NP_001018008 NP_001029245 NP_001029246 NP_001029247 NP_001157724