BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4996

Title: NMR-Based Structure of the Conserved Protein MTH865 from the Archea Methanobacterium thermoautotrophicum

Authors: Lee, Gregory; Edwards, Aled; Arrowsmith, Cheryl; McIntosh, Lawrence

Citation: Lee, Gregory; Edwards, Aled; Arrowsmith, Cheryl; McIntosh, Lawrence. "Letter to the Editor: NMR-Based Structure of the Conserved Protein MTH865 from the Archaeon Methanobacterium thermoautotrophicum"  J. Biomol. NMR 21, 63-66 (2001).

Assembly members:
Methanobacterium thermoautotrophicum open reading frame 865, polymer, 84 residues, 8675 Da.

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanothermobacter thermautotrophicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Methanobacterium thermoautotrophicum open reading frame 865: GSHMKMGVKEDIRGQIIGAL AGADFPINSPEELMAALPNG PDTTCKSGDVELKASDAGQV LTADDFPFKSAEEVADTIVN KAGL

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts540
13C chemical shifts324
15N chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MTH8651

Entities:

Entity 1, MTH865 84 residues - 8675 Da.

1   GLYSERHISMETLYSMETGLYVALLYSGLU
2   ASPILEARGGLYGLNILEILEGLYALALEU
3   ALAGLYALAASPPHEPROILEASNSERPRO
4   GLUGLULEUMETALAALALEUPROASNGLY
5   PROASPTHRTHRCYSLYSSERGLYASPVAL
6   GLULEULYSALASERASPALAGLYGLNVAL
7   LEUTHRALAASPASPPHEPROPHELYSSER
8   ALAGLUGLUVALALAASPTHRILEVALASN
9   LYSALAGLYLEU

Samples:

15N-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-100% 15N], 0.5 mM

13C_15N-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-100% 13C; U-100% 15N], 0.5 mM

10%_13C-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-10% 13C; U-100% 15N], 0.5 mM

condition_1: pH: 6.51; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
500MHz Unity housed at Univ. of British Columbianot availablenot availablecondition_1
600MHz INOVA housed at Univ. of Torontonot availablenot availablecondition_1
800MHz INOVA housed at Univ. of Albertanot availablenot availablecondition_1

Software:

Felix v2000 - raw data processing, peak picking, peak assignments

NMR spectrometers:

  • Varian UNITY 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAM70032
EMBL CAA48863 CAA48865
GB AAB85363
REF NP_276002 WP_010876498