BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5006

Title: 1H Chemical Shift Assignments for alpha-Bungarotoxin   PubMed: 11814338

Authors: Scarselli, Maria; Spiga, Ottavia; Ciutti, Arianna; Bernini, Andrea; Bracci, Luisa; Lelli, Barbara; Lozzi, Luisa; Calamandrei, Duccio; Di Maro, Daniela; Klein, Samuel; Niccolai, Neri

Citation: Scarselli, Maria; Spiga, Ottavia; Ciutti, Arianna; Bernini, Andrea; Bracci, Luisa; Lelli, Barbara; Lozzi, Luisa; Calamandrei, Duccio; Di Maro, Daniela; Klein, Samuel; Niccolai, Neri. "NMR Structure of Alpha-bungarotoxin free and bound to a Mimotope of the Nicotinic Acetylcholine Receptor"  Biochemistry 41, 1457-1463 (2002).

Assembly members:
alpha-bungarotoxin, polymer, 74 residues, 7984 Da.

Natural source:   Common Name: many-banded krait   Taxonomy ID: 8616   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: bungarus multicinctus

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
alpha-bungarotoxin: IVCHTTATSPISAVTCPPGE NLCYRKMWCDAFCSSRGKVV ELGCAATCPSKKPYEEVTCC STDKCNPHPKQRPG

Data sets:
Data typeCount
1H chemical shifts447

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-btx1

Entities:

Entity 1, alpha-btx 74 residues - 7984 Da.

1   ILEVALCYSHISTHRTHRALATHRSERPRO
2   ILESERALAVALTHRCYSPROPROGLYGLU
3   ASNLEUCYSTYRARGLYSMETTRPCYSASP
4   ALAPHECYSSERSERARGGLYLYSVALVAL
5   GLULEUGLYCYSALAALATHRCYSPROSER
6   LYSLYSPROTYRGLUGLUVALTHRCYSCYS
7   SERTHRASPLYSCYSASNPROHISPROLYS
8   GLNARGPROGLY

Samples:

sample_1: alpha-bungarotoxin 0.5 mM

Ex-cond_1: pH: 5.67; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H TOCSYnot availablenot availablenot available
1H NOESYnot availablenot availablenot available

Software:

XwinNMR v2.1 - spectra acquisition and processing

NMRView v4.1 - peaks assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15130 5024 8
PDB
EMBL CAA63045 CAB50692 CAB51841 CAB51842 CAB51843
GB AAC83981 AAC83982 AAC83983 AAC83984 AAC83985
SP P60615 P60616 Q7T3J2