BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5010

Title: 1H, 15N, and 13C chemical shift assignments for DNA polymerase X

Authors: Maciejewski, Mark; Pan, Borlan; Shin, Ronald; Denninger, Adam; Mullen, Gregory

Citation: Maciejewski, Mark; Pan, Borlan; Shin, Ronald; Denninger, Adam; Mullen, Gregory. "Letter to the Editor: 1H, 15N, and 13C Resonance Assignments for a 20 kDa DNA Polymerase from African Swine Fever Virus"  J. Biomol. NMR 21, 177-178 (2001).

Assembly members:
DNA Polymerase X, polymer, 174 residues, 20314 Da.

Natural source:   Common Name: African swine fever virus BA71V   Taxonomy ID: 10498   Superkingdom: not available   Kingdom: not available   Genus/species: African swine fever-like viruses African swine fever virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNA Polymerase X: MLTLIQGKKIVNHLRSRLAF EYNGQLIKILSKNIVAVGSL RREEKMLNDVDLLIIVPEKK LLKHVLPNIRIKGLSFSVKV CGERKCVLFIEWEKKTYQLD LFTALAEEKPYAIFHFTGPV SYLIRIRAALKKKNYKLNQY GLFKNQTLVPLKITTEKELI KELGFTYRIPKKRL

Data sets:
Data typeCount
1H chemical shifts1213
13C chemical shifts725
15N chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pol X1

Entities:

Entity 1, Pol X 174 residues - 20314 Da.

1   METLEUTHRLEUILEGLNGLYLYSLYSILE
2   VALASNHISLEUARGSERARGLEUALAPHE
3   GLUTYRASNGLYGLNLEUILELYSILELEU
4   SERLYSASNILEVALALAVALGLYSERLEU
5   ARGARGGLUGLULYSMETLEUASNASPVAL
6   ASPLEULEUILEILEVALPROGLULYSLYS
7   LEULEULYSHISVALLEUPROASNILEARG
8   ILELYSGLYLEUSERPHESERVALLYSVAL
9   CYSGLYGLUARGLYSCYSVALLEUPHEILE
10   GLUTRPGLULYSLYSTHRTYRGLNLEUASP
11   LEUPHETHRALALEUALAGLUGLULYSPRO
12   TYRALAILEPHEHISPHETHRGLYPROVAL
13   SERTYRLEUILEARGILEARGALAALALEU
14   LYSLYSLYSASNTYRLYSLEUASNGLNTYR
15   GLYLEUPHELYSASNGLNTHRLEUVALPRO
16   LEULYSILETHRTHRGLULYSGLULEUILE
17   LYSGLULEUGLYPHETHRTYRARGILEPRO
18   LYSLYSARGLEU

Samples:

Pol_X_sample_1: DNA Polymerase X, [U-13C; U-15N], 0.6 – 1.0 mM; PIPES 20 mM; NaCl 500 mM; DTT 10 mM; AEBSF 0.5 mM; sodium azide 0.02%

Pol_X_sample_2: DNA Polymerase X, [U-15N], 0.6 – 1.0 mM; PIPES 20 mM; NaCl 500 mM; DTT 10 mM; AEBSF 0.5 mM; sodium azide 0.02%

Pol_X_sample_3: DNA Polymerase X0.6 – 1.0 mM; PIPES 20 mM; NaCl 500 mM; DTT 10 mM; AEBSF 0.5 mM; sodium azide 0.02%

Pol_X_conditions_1: pH: 6.5; temperature: 298 K; ionic strength: 500 mM

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablePol_X_conditions_1
15N-edited NOESY-HSQCnot availablenot availablePol_X_conditions_1
HNCAnot availablenot availablePol_X_conditions_1
HN(CO)CAnot availablenot availablePol_X_conditions_1
HNCACBnot availablenot availablePol_X_conditions_1
CBCA(CO)NHnot availablenot availablePol_X_conditions_1
HNCOnot availablenot availablePol_X_conditions_1
HNHAnot availablenot availablePol_X_conditions_1
HNHBnot availablenot availablePol_X_conditions_1
HCC(CO)NH-TOCSYnot availablenot availablePol_X_conditions_1
CC(CO)NH-TOCSYnot availablenot availablePol_X_conditions_1
HCCH-TOCSYnot availablenot availablePol_X_conditions_1
1H-13C HSQCnot availablenot availablePol_X_conditions_1
13C-edited NOESY-HSQCnot availablenot availablePol_X_conditions_1
13C-edited HSQC-NOESYnot availablenot availablePol_X_conditions_1
2D TOCSYnot availablenot availablePol_X_conditions_1
2D NOESYnot availablenot availablePol_X_conditions_1

Software:

nmrPipe - NMR data procesing

XEASY - NMR data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18933 18934 18935
PDB
EMBL CAN10196 CAN10446 CBH29197
GB AAA65326 AIY22288 AIY22446
PRF 2113434DC
REF NP_042790
SP P0C984 P42494