BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5012

Title: Gelatin-binding Region of Human Matrix Metalloproteinase-2: Solution Structure, Dynamics, and Function of the COL-23 Two-domain Construct   PubMed: 11320090

Authors: Briknarova, Klara; Gehrmann, Marion; Banyai, Laszlo; Tordai, Hedwig; Patthy, Laszlo; Llinas, Miguel

Citation: Briknarova, Klara; Gehrmann, Marion; Banyai, Laszlo; Tordai, Hedwig; Patthy, Laszlo; Llinas, Miguel. "Gelatin-binding Region of Human Matrix Metalloproteinase-2: Solution Structure, Dynamics, and Function of the COL-23 Two-domain Construct"  J. Biol. Chem. 276, 27613-27621 (2001).

Assembly members:
Matrix Metalloproteinase 2, polymer, 72 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Matrix Metalloproteinase 2: LAAHPPFASWMSTVGGNSGG APCVFPFTFLGNKYESCTSA GRSDGKMWCATTANYDDDRK WGFCPDQGWISS

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts75
1H chemical shifts424

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1col-3 domain, MMP-21

Entities:

Entity 1, col-3 domain, MMP-2 72 residues - Formula weight is not available

1   LEUALAALAHISPROPROPHEALASERTRP
2   METSERTHRVALGLYGLYASNSERGLYGLY
3   ALAPROCYSVALPHEPROPHETHRPHELEU
4   GLYASNLYSTYRGLUSERCYSTHRSERALA
5   GLYARGSERASPGLYLYSMETTRPCYSALA
6   THRTHRALAASNTYRASPASPASPARGLYS
7   TRPGLYPHECYSPROASPGLNGLYTRPILE
8   SERSER

Samples:

sample_1: Matrix Metalloproteinase 2 0.5 mM

sample_3: Matrix Metalloproteinase 2, [U-15N], 1 mM

Ex-cond_1: pH: 5.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 15N NOESYnot availablenot availablenot available
3D 15N TOCSYnot availablenot availablenot available
HNHBnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

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