BMRB Entry 50130

Title:
Backbone assignments of Tau fragment (225-324) with P301L mutation
Deposition date:
2019-12-15
Original release date:
2020-08-03
Authors:
Kawasaki, Ryosuke
Citation:

Citation: Kawasaki, Ryosuke; Tate, Shin-Ichi. "Impact of the Hereditary P301L Mutation on the Correlated Conformational Dynamics of Human Tau Protein Revealed by the Paramagnetic Relaxation Enhancement NMR Experiments"  Int. J. Mol. Sci. 21, .-. (2020).
PubMed: 32486218

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts101
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P301L TauF4d fragment1

Entities:

Entity 1, P301L TauF4d fragment 104 residues - Formula weight is not available

1   GLYSERHISMETLYSVALALAVALVALARG
2   THRPROPROLYSSERPROSERSERALALYS
3   SERARGLEUGLNTHRALAPROVALPROMET
4   PROASPLEULYSASNVALLYSSERLYSILE
5   GLYSERTHRGLUASNLEULYSHISGLNPRO
6   GLYGLYGLYLYSVALGLNILEILEASNLYS
7   LYSLEUASPLEUSERASNVALGLNSERLYS
8   CYSGLYSERLYSASPASNILELYSHISVAL
9   LEUGLYGLYGLYSERVALGLNILEVALTYR
10   LYSPROVALASPLEUSERLYSVALTHRSER
11   LYSCYSGLYSER

Samples:

sample_1: P301L TauF4d fragment, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS 50 mM; DTT 1 mM; sodium azide 0.03%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (H)CC(CO)NNHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks