BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5024

Title: The NMR solution structure of alpha-bungarotoxin   PubMed: 11312275

Authors: Zeng, Haoyu; Moise, Leonard; Grant, Marianne; Hawrot, Edward

Citation: Zeng, Haoyu; Moise, Leonard; Grant, Marianne; Hawrot, Edward. "The Solution Structure of the Complex Formed between alpha-Bungarotoxin and an 18mer Cognate Peptide Derived from the alpha1 Subunit of the Nicotinic Acetylcholine Receptor from Torpedo californica"  J. Biol. Chem. 276, 22930-22940 (2001).

Assembly members:
alpha-neurotoxin, polymer, 74 residues, 7977 Da.

Natural source:   Common Name: Many-banded krait   Taxonomy ID: 8616   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bungarus multicinctus

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
alpha-neurotoxin: IVCHTTATSPISAVTCPPGE NLCYRKMWCDAFCSSRGKVV ELGCAATCPSKKPYEEVTCC STDKCNPHPKQRPG

Data sets:
Data typeCount
1H chemical shifts298

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alpha-Bungarotoxin1

Entities:

Entity 1, Alpha-Bungarotoxin 74 residues - 7977 Da.

1   ILEVALCYSHISTHRTHRALATHRSERPRO
2   ILESERALAVALTHRCYSPROPROGLYGLU
3   ASNLEUCYSTYRARGLYSMETTRPCYSASP
4   ALAPHECYSSERSERARGGLYLYSVALVAL
5   GLULEUGLYCYSALAALATHRCYSPROSER
6   LYSLYSPROTYRGLUGLUVALTHRCYSCYS
7   SERTHRASPLYSCYSASNPROHISPROLYS
8   GLNARGPROGLY

Samples:

sample_1: alpha-neurotoxin 2.0 mM; potassium acetate, [U-2H], 50 mM; H2O 95%; D2O 5%; sodium azide 0.05%

sample_cond_1: pH: 4.0; temperature: 308 K; ionic strength: 28.7 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

XWINNMR v2.1 - collection

NMRPipe v1.8 - processing

Sparky v3.95 - data analysis

CNSsolve v1.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 15130 5006 8
PDB
EMBL CAA63045 CAB50692 CAB51841 CAB51842 CAB51843
GB AAC83981 AAC83982 AAC83983 AAC83984 AAC83985
SP P60615 P60616 Q7T3J2