BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5027

Title: NMR Structure of Human Fibronectin EDA   PubMed: 11775745

Authors: Niimi, Tatsuya; Osawa, Masanori; Yamaji, Noboru; Yasunaga, Kunio; Sakashita, Hitoshi; Mase, Toshiyasu; Tanaka, Akihiro; Fujita, Shigeo

Citation: Niimi, Tatsuya; Osawa, Masanori; Yamaji, Noboru; Yasunaga, Kunio; Sakashita, Hitoshi; Mase, Toshiyasu; Tanaka, Akihiro; Fujita, Shigeo. "NMR Structure of Human Fibronectin EDA"  J. Biomol. NMR 21, 281-284 (2001).

Assembly members:
human fibronectin EDA, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
human fibronectin EDA: NIDRPKGLAFTDVDVDSIKI AWESPQGQVSRYRVTYSSPE DGIHELFPAPDGEEDTAELQ GLRPGSEYTVSVVALHDDME SQPLIGTQSTAIPA

Data sets:
Data typeCount
1H chemical shifts619
13C chemical shifts392
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human fibronectin EDA1

Entities:

Entity 1, human fibronectin EDA 94 residues - Formula weight is not available

1   ASNILEASPARGPROLYSGLYLEUALAPHE
2   THRASPVALASPVALASPSERILELYSILE
3   ALATRPGLUSERPROGLNGLYGLNVALSER
4   ARGTYRARGVALTHRTYRSERSERPROGLU
5   ASPGLYILEHISGLULEUPHEPROALAPRO
6   ASPGLYGLUGLUASPTHRALAGLULEUGLN
7   GLYLEUARGPROGLYSERGLUTYRTHRVAL
8   SERVALVALALALEUHISASPASPMETGLU
9   SERGLNPROLEUILEGLYTHRGLNSERTHR
10   ALAILEPROALA

Samples:

sample_1: human fibronectin EDA, [U-15N; U-13C], 2.0 mM; sodium phosphate 20 mM; Na2SO4 400 mM

cond_set_1: pH: 7.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C CT-HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CBCACO)NHnot availablenot availablenot available
3D H(CCO)NHnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D 13C-edited NOESY-HSQCnot availablenot availablenot available
3D 15N-edited NOESY-HSQCnot availablenot availablenot available
3D HNHAnot availablenot availablenot available

Software:

NMRpipe - Data processing

PIPP - Peak picking

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
EMBL CAB52436
GB AAA67748